Stable isotope aided nuclear magnetic resonance study to investigate the receptor-binding site of human interleukin 1. beta

Journal Article · Tue Mar 03 00:00:00 EST 1992 · Biochemistry; (United States)

DOI:https://doi.org/10.1021/bi00123a032· OSTI ID:5562592

Tate, Shinichi; Ichikawa, Saori; Kaneko, Midori; Inagaki, Fuyuhiko ^[1]; Kikumoto, Yoshikazu; Masui, Yoshihiro; Kamogashira, Takashi ^[2]; Ouchi, Muneki ^[3]; Takahashi, Seizo ^[4]

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1. Tokyo Metropolitan Inst. of Medical Science (Japan)
2. Otsuka Pharmaceutical Co., Ltd., Tokushima (Japan)
3. JEOL, Inc., Tokyo (Japan)
4. Japan Womens College, Tokyo (Japan)

Resonance assignments for interleukin 1{beta} at neutral pH were made by using three-dimensional NMR in combination with specific labeling and double-labeling methods with stable isotopes. On the basis of the present assignments, {sup 15}N single-quantum coherence spectra of N-terminal truncated and fusion mutants were compared with that of the wild-type. Although these mutants have reduced biological activity, they showed {sup 15}N-SQC spectra similar to that of the wild-type. However, small but significant chemical shift changes were observed for amino acid residues within a loop 86-99, in spite of the modification at the N-terminus, supporting the idea that this loop forms a biologically active part of interleukin 1{beta}. Receptor-binding activity was studied for mutants (Asp-93)-, (Leu-93)-, and des-(Arg-98)interleukin 1{beta}'s. The results show significant loss of the receptor-binding activity. The N-terminus, the C-terminus, and the loop 86-99 form a part of the open end of a {beta}-barrel which forms the receptor-binding site of IL-1{beta}.

• OSTI ID: 5562592
• Journal Information: Biochemistry; (United States), Vol. 31:8; ISSN 0006-2960
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
LYMPHOKINES
NUCLEAR MAGNETIC RESONANCE
RECEPTORS
MOLECULAR STRUCTURE
CARBON 13
INFLAMMATION
NITROGEN 15
CARBON ISOTOPES
EVEN-ODD NUCLEI
GROWTH FACTORS
ISOTOPES
LIGHT NUCLEI
MAGNETIC RESONANCE
MEMBRANE PROTEINS
MITOGENS
NITROGEN ISOTOPES
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PATHOLOGICAL CHANGES
PROTEINS
RESONANCE
STABLE ISOTOPES
SYMPTOMS
550201* - Biochemistry- Tracer Techniques