Content and vacuole extravacuole distribution of neutral sugars, free amino acids, and anthocyanin in protoplasts
Neutral sugar, free amino acid, and anthocyanin levels and vacuole/extravacuole distribution were determined for Hippeastrum and Tulipa petal and Tulipa leaf protoplasts. Glucose and fructose, the predominant neutral monosaccharides observed, were primarily vacuolar in location. Glutamine, the predominant free amino acid found, was primarily extravacuolar. ..gamma..-methyleneglutamate was identified as a major constituent of Tulipa protoplasts. Qualitative characterization of Hippeastrum petal and vacuole organic acids indicated the presence of oxalic, malic, citric, and isocitric acids. Data are presented which indicate that vacuoles obtained by gentle osmotic shock of protoplasts in dibasic phosphate have good purity and retain their contents.
- Research Organization:
- Brookhaven National Lab. (BNL), Upton, NY (United States)
- OSTI ID:
- 5558471
- Journal Information:
- Plant Physiol.; (United States), Vol. 64:1
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
OXALIC ACID
AMINO ACIDS
CITRIC ACID
FRUCTOSE
GLUCOSE
KREBS CYCLE
LEAVES
MALEIC ACID
ORGANIC ACIDS
PIGMENTS
PLANTS
SACCHAROSE
TISSUE DISTRIBUTION
ALDEHYDES
CARBOHYDRATES
CARBOXYLIC ACIDS
DICARBOXYLIC ACIDS
DISACCHARIDES
DISTRIBUTION
HEXOSES
HYDROXY ACIDS
KETONES
MONOSACCHARIDES
OLIGOSACCHARIDES
ORGANIC COMPOUNDS
SACCHARIDES
550300* - Cytology