The role of Kupffer cells in glucan-induced granuloma formation in the liver of mice depleted of blood monocytes by administration of strontium-89

Journal Article · Wed May 01 00:00:00 EDT 1991 · Laboratory Investigation (A Journal of Experimental Methods and Pathology); (USA)

OSTI ID:5545236

Naito, M; Takahashi, K ^[1]

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1. Kumamoto Univ. Medical School, (Japan)

In order to elucidate the role of Kupffer cells in granuloma formation in the liver of mice under a condition of severe monocytopenia induced by administration of strontium-89, granulomas were produced by particulate glucan injection and examined histopathologically, immunohistochemically, by ({sup 3}H)thymidine autoradiography, and in culture experiments. Hepatic granulomas were smaller, less numerous, and more irregularly shaped in the monocytopenic mice than in the control mice. The granulomas were composed of multinuclear giant cells, epithelioid cells, Kupffer cells, and T lymphocytes, but not monocytes or granulocytes. Kupffer cells were heavily labeled with ({sup 3}H)thymidine in the monocytopenic mice, particularly just before the stage of granuloma formation, and then clustered in the liver sinusoids. At 8 days, they formed granulomas, transformed into epithelioid cells, and transformed further into multinuclear giant cells. Although the culture of liver cell suspensions prepared from the livers of monocytopenic mice sustained diffuse proliferation of macrophages on a monolayer of mouse stromal cell line (ST2), no monocyte/macrophage colonies were formed. From these results, it is reasonable to conclude that Kupffer cells alone are activated in a condition without a supply of monocytes from peripheral blood; proliferate and cluster in the hepatic sinusoids; transform into peroxidase-negative macrophages, epithelioid cells, and multinuclear giant cells; and participate in granuloma formation in loco together with T lymphocytes.

• OSTI ID: 5545236
• Journal Information: Laboratory Investigation (A Journal of Experimental Methods and Pathology); (USA), Vol. 64:5; ISSN 0023-6837
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
LIVER
GRANULOMAS
MONOCYTES
CELL PROLIFERATION
RETICULOENDOTHELIAL SYSTEM
BIOLOGICAL FUNCTIONS
BIOLOGICAL RADIATION EFFECTS
BLOOD COUNT
CELL DIVISION
DNA
LYMPHOCYTES
MACROPHAGES
MICE
STRONTIUM 90
THYMIDINE
TRACER TECHNIQUES
TRITIUM COMPOUNDS
ALKALINE EARTH ISOTOPES
ANIMAL CELLS
ANIMAL TISSUES
ANIMALS
AZINES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
CONNECTIVE TISSUE CELLS
DIGESTIVE SYSTEM
DISEASES
EVEN-EVEN NUCLEI
GLANDS
HETEROCYCLIC COMPOUNDS
HYDROGEN COMPOUNDS
INTERMEDIATE MASS NUCLEI
ISOTOPE APPLICATIONS
ISOTOPES
LEUKOCYTES
MAMMALS
MATERIALS
NEOPLASMS
NUCLEI
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PHAGOCYTES
PYRIMIDINES
RADIATION EFFECTS
RADIOISOTOPES
RIBOSIDES
RODENTS
SOMATIC CELLS
STRONTIUM ISOTOPES
TISSUES
VERTEBRATES
YEARS LIVING RADIOISOTOPES
550901* - Pathology- Tracer Techniques
560162 - Radionuclide Effects
Kinetics
& Toxicology- Animals
Plants
Microorganisms
& Cells