Restoration of lymphocyte proliferation and CTL generation by murine rIL-2 after treatment of allogeneic stimulator cells by ultraviolet B irradiation, heat, or paraformaldehyde
- Washington Univ. School of Medicine, St. Louis, Mo (USA)
Following a 5-day mixed lymphocyte culture (MLC), C3H/HeJ (H-2k) splenocytes stimulated with DBA/2 (H-2d) gamma-irradiated splenocytes (2000 rads) are specifically cytotoxic in a 4-hr {sup 51}Cr-release assay to P815 (H-2d) target cells (62 +/- 2% cytolysis) but not to third-party EL4 (H-2b). However, when the DBA/2 stimulator cells were treated with heat inactivation (45{degree}C for 1 hr), fixed with 1% paraformaldehyde (15 min), or irradiated with ultraviolet-B light (10(4) J/M2), no cell proliferation or cytolytic activity developed in the MLCs. The levels of IL-1, IL-2, and IL-6 from the supernatants of MLC using stimulators undergoing either of the three treatments were markedly decreased compared with that from gamma-irradiated stimulators. Both cell proliferation and specific cytolysis were restored in a dose-dependent fashion by the addition of murine rIL-2 to the MLCs. If the stimulator cells were first activated with 5 micrograms/ml pokeweed mitogen or lipopolysaccharide for 2 days, the subsequent treatment with heat, paraformaldehyde, or UV-B did not significantly affect the development of cytolysis (54-70% cytolysis). Suppressor cells were not detected when cells from the nonresponsive MLCs (2.5 x 10(6) cells) were added to an MLC freshly prepared with gamma-irradiated stimulator cells, or were injected intraperitoneally (50 x 10(6) cells) into naive mice 2 days before recovery and in vitro sensitization of splenocytes. Therefore, modification of the stimulating alloantigen can prevent the release of cytokines that function as an essential second signal in the development of the proliferative response and subsequent cytolysis. The cytokine found to be essential for restoration of this response is IL-2.
- OSTI ID:
- 5545216
- Journal Information:
- Transplantation; (USA), Vol. 51:5; ISSN 0041-1337
- Country of Publication:
- United States
- Language:
- English
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FORMALDEHYDE
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LYMPHOKINES
BIOSYNTHESIS
BIOLOGICAL RADIATION EFFECTS
CHROMIUM 51
DOSE-RESPONSE RELATIONSHIPS
GAMMA RADIATION
MICE
SPLEEN CELLS
TRACER TECHNIQUES
ULTRAVIOLET RADIATION
ALDEHYDES
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BIOLOGICAL MATERIALS
BLOOD
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BODY FLUIDS
CHROMIUM ISOTOPES
CONNECTIVE TISSUE CELLS
DAYS LIVING RADIOISOTOPES
ELECTROMAGNETIC RADIATION
ELECTRON CAPTURE RADIOISOTOPES
ENERGY
EVEN-ODD NUCLEI
GROWTH FACTORS
INTERMEDIATE MASS NUCLEI
IONIZING RADIATIONS
ISOTOPE APPLICATIONS
ISOTOPES
LEUKOCYTES
MAMMALS
MATERIALS
MITOGENS
NUCLEI
ORGANIC COMPOUNDS
PROTEINS
RADIATION EFFECTS
RADIATIONS
RADIOISOTOPES
RODENTS
SOMATIC CELLS
SYNTHESIS
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560300* - Chemicals Metabolism & Toxicology
560120 - Radiation Effects on Biochemicals
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