Immobilization of stationary phases onto chromatographic supports by gamma radiation
Cobalt-60 gamma radiation has been found to be an effective means of immobilizing stationary phases onto gas and liquid chromatographic supports. The ability of a phase to crosslink was determined to be highly dependent on its chemical structure. Gamma radiation was employed to immobilize Se-30, a methylsilicone, and 216 PS onto Chromosorb WHP for packed column chromatography. The resultant columns exhibited increased thermal stability and a high degree of efficiency. Several blended phases containing varying ratios of Se-30:216 PS were also immobilized onto chromatographic packings. This work was further extended to the immobilization of Se-30 and 216 PS onto fused silica capillaries via gamma radiation. Columns exhibited excellent efficiency and improved thermal stability. An investigation into the role of immobilization of polymeric phases for HPLC was undertaken. Results indicate that gamma radiation can immobilzied polyoctadecylsiloxane and polyoctylsiloxane onto silica surfaces. ESCA and elemental analyses were performed to gain a better understanding of the surface and bulk content of immobilized HPLC packings.
- Research Organization:
- Lowell Univ., MA (USA)
- OSTI ID:
- 5523805
- Resource Relation:
- Other Information: Thesis (Ph. D.)
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
SILICON
CHEMICAL RADIATION EFFECTS
PHASE STUDIES
COBALT 60
CROSS-LINKING
GAMMA RADIATION
GAS CHROMATOGRAPHY
LIQUID COLUMN CHROMATOGRAPHY
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CHEMICAL REACTIONS
CHEMISTRY
CHROMATOGRAPHY
COBALT ISOTOPES
ELECTROMAGNETIC RADIATION
ELEMENTS
INTERMEDIATE MASS NUCLEI
INTERNAL CONVERSION RADIOISOTOPES
IONIZING RADIATIONS
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
MINUTES LIVING RADIOISOTOPES
NUCLEI
ODD-ODD NUCLEI
POLYMERIZATION
RADIATION CHEMISTRY
RADIATION EFFECTS
RADIATIONS
RADIOISOTOPES
SEMIMETALS
SEPARATION PROCESSES
YEARS LIVING RADIOISOT
400600* - Radiation Chemistry