Radioimmunoassay of progesterone in saliva: a simplified technique using /sup 125/I-radioligand
The value of serial salivary progesterone (P) determinations for evaluation of corpus luteum function compared with sporadic serum samplings is recognized. Salivary P concentrations have previously been determined by a relatively difficult assay which utilizes a tritiated radioligand. The present study was designed to compare this conventional method with a simpler assay utilizing an iodinated radioligand. The values obtained with two different radioimmunoassay (RIA) systems were compared for samples collected throughout the luteal phase of seven normal menstrual cycles and three conception cycles. In the seven nonconception luteal phases as well as in the three conception luteal phases, daily salivary P determinations by the two techniques correlated significantly with each other (r = 0.56; P less than 0.001). For the seven nonconception luteal phases, the salivary P determinations obtained by /sup 125/I RIA (Saliva /sup 125/I) correlated significantly with the matched serum values (r = 0.54; P less than 0.001). However, the correlation was poor between the salivary P determinations obtained by the /sup 3/H RIA (Saliva /sup 3/H) and the matched serum values. The simplicity and accuracy of the /sup 125/I RIA and the convenience of saliva sampling provide a practical approach to monitor human luteal function.
- Research Organization:
- Medical College of Georgia, Augusta
- OSTI ID:
- 5520667
- Journal Information:
- Fert. Steril.; (United States), Vol. 43:3
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
PROGESTERONE
RADIOIMMUNOASSAY
COMPARATIVE EVALUATIONS
IODINE 125
LABELLED COMPOUNDS
LIGANDS
SALIVA
TRACER TECHNIQUES
TRITIUM COMPOUNDS
BETA DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BODY FLUIDS
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
HORMONES
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KETONES
MATERIALS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PREGNANES
RADIOASSAY
RADIOISOTOPES
STEROID HORMONES
STEROIDS
550201* - Biochemistry- Tracer Techniques