Biophysical applications of solid state and tritium NMR

Thesis/Dissertation · Thu Jan 01 00:00:00 EST 1987

OSTI ID:5517008

Un, S

Novel applications of Magic Angle Sample Spinning (MASS) and Tritium ({sup 3}H) NMR to the study of mammalian tissue and in vivo metabolism, respectively, are described. The solid state {sup 31} P MASS NMR spectra of sodium, magnesium and calcium complexes of adenosine triphosphate (ATP) were recorded. These spectra indicate that the terminal phosphate group of the sodium complex is protonated in contrast to the unprotonated divalent complexes. The inductive effects of the divalent counter ions are also discussed. To better understand the nature of the {sup 31}P chemical shift interaction, the chemical shift tensors of a large variety of phosphates and phosphate esters were measured. The magnitude of the {sigma}{sub 33}, the low field, elements of the {sup 31}P chemical shift tensors in these phosphates were linearly related to the longest P-O bonds and the {simga}{sub 11}, the high field, elements to the shortest bonds. Similar relationships were found when these tensor elements were plotted against bond angles. The implications of these observations are discussed in the context of phosphorus-oxygen d-p {kappa} bonding interactions. The metabolism of (1-{sup 3}h) glucose in human and rat erythrocytes was monitored by {sup 3}H NMR.

• Research Organization: California Univ., Berkeley, CA (USA)
• OSTI ID: 5517008
• Resource Relation: Other Information: Thesis (Ph. D.)
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
ATP
NMR SPECTRA
GLUCOSE
METABOLISM
CHEMICAL SHIFT
ERYTHROCYTES
MAN
NUCLEAR MAGNETIC RESONANCE
PHOSPHATES
PHOSPHORUS 31
RATS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
ALDEHYDES
ANIMALS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARBOHYDRATES
HEXOSES
HYDROGEN COMPOUNDS
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
MAGNETIC RESONANCE
MAMMALS
MATERIALS
MONOSACCHARIDES
NUCLEI
NUCLEOTIDES
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
OXYGEN COMPOUNDS
PHOSPHORUS COMPOUNDS
PHOSPHORUS ISOTOPES
PRIMATES
RESONANCE
RODENTS
SACCHARIDES
SPECTRA
STABLE ISOTOPES
VERTEBRATES
550201* - Biochemistry- Tracer Techniques
550501 - Metabolism- Tracer Techniques