Viruses of eukaryotice green algae
The primary objective of our research was to develop the Chlorella-PBCV-1 virus system so that it can be used as a model system for studying gene expression in a photosynthetic eukaryote. We have made considerable progress and have learned much about PBCV-1 and its replication cycle. In addition, several significant discoveries were made in the last 3 to 4 years. These discoveries include: (i) the finding that morphologically similar, plaque forming, dsDNA containing viruses are common in nature and can be isolated readily from fresh water, (ii) the finding that all of these Chlorella viruses contain methylated bases which range in concentration from 0.1% to 47.5% m{sup 5}dC and 0 to 37% m{sup 6}dA and (iii) the discovery that infection with at least some of these viruses induces the appearance of DNA modification/restriction systems. 26 refs.
- Research Organization:
- Nebraska Univ., Lincoln, NE (USA). Dept. of Plant Pathology
- Sponsoring Organization:
- DOE/ER
- DOE Contract Number:
- AC02-82ER12086
- OSTI ID:
- 5516818
- Report Number(s):
- DOE/ER/12086-2; ON: DE89017811
- Country of Publication:
- United States
- Language:
- English
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