Anaphylatoxin C5a fails to promote prostacyclin release from cultured human endothelial cells
A predominantly relaxing effect of C5a on isolated blood vessels has been reported, which is associated with prostacyclin release from the vessel wall. Further, the well known hypothensive effect of C5a, also associated with increased prostacyclin output and preventable by indomethacin, indicates an involvement of endothelial cells in this reaction. In this study the authors characterized the response to C5a of cultured human endothelial cells from umbilical vein as measured by prostacyclin release. Prostacyclin was quantitated by radioimmunoassay as 6-keto-PGF/sub 1..cap alpha../. Subcultured cells respond to histamine and mellitin with increased prostacyclin production, but do not respond to leukotriene C4 (LTC/sub 4/). Primary cultures, on the other hand, respond to LTC/sub 4/ and the histamine response is 7-fold greater for these cells than for subcultured cells. However, neither primary nor subcultured cells release prostacyclin following application of either human C5a (100 nM) or C3a (1 ..mu..M). Also, these cells fail to show specific binding sites for /sup 125/I-C5a. In contrast, endothelial cells in the presence of human PMNs challenged with C5a release prostacyclin. These data suggest that C5a has no direct effect on the endothelial cell, but rather activates this cell indirectly via mediators from other cells known to respond to C5a.
- Research Organization:
- Scripps Clinic and Research Foundation, La Jolla, CA
- OSTI ID:
- 5497590
- Report Number(s):
- CONF-8604222-; TRN: 86-026591
- Journal Information:
- Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 45:3; Conference: 70. annual meeting of the Federation of American Society for Experimental Biology, St. Louis, MO, USA, 13 Apr 1986
- Country of Publication:
- United States
- Language:
- English
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