Human platelets labeled with In-111 8-hydroxyquinoline: kinetics, distribution, and estimates of radiation dose
Platelets from nine normal male subjects were labeled with In-111 8-hydroxyquinoline (In-111 oxine) in the presence of plasma in either ''closed'' blood transfer packs or in ''open'' test tubes. The mean labeling efficiencies in these two systems were 27 and 53%, respectively. Mean survival time of In-111-labeled autologous platelets was 8.76 days, with a standard deviation of 1.05 according to the maximum-likelihood estimate of the gamma-function model. The initial recovery of In-111 platelets in the circulation was 57% with a standard deviation of 11%. The distribution of In-111 platelets in liver and spleen was quantitated by anterior, posterior, and transmission gamma-camera imaging. During the first 30 min, 38% of the injected dose accumulated in the spleen, 13% in the liver. No significant increase in In-111 radioactivity was observed in either of the two organs over a 3-9-day period. The bone marrow was an additional site of In-111 accumulation. The spleen was the critical organ with respect to radiation dose. The splenic dose was estimated to be 34 rad/mCi In-111 platelets, that of the liver 2.1 rad/mCi. With the injection of 100-150 microCi of In-111-labeled platelets in normal subjects, giving a splenic radiation of 5 rad, a complete 10-day survival study can be performed and uptake of In-111 in different organs can be measured quantitatively for at least 3-4 days.
- Research Organization:
- The Johns Hopkins Medical Institutions, Baltimore, Maryland
- OSTI ID:
- 5474628
- Journal Information:
- J. Nucl. Med.; (United States), Vol. 23:2
- Country of Publication:
- United States
- Language:
- English
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BLOOD PLATELETS
RADIONUCLIDE KINETICS
TISSUE DISTRIBUTION
BONE MARROW
RADIATION DOSES
INDIUM 111
LIVER
SPLEEN
MEN
OXINE
ANIMAL TISSUES
ANIMALS
AZINES
BETA DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
DISTRIBUTION
DOSES
ELECTRON CAPTURE RADIOISOTOPES
GLANDS
HEMATOPOIETIC SYSTEM
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
INDIUM ISOTOPES
INTERMEDIATE MASS NUCLEI
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
MALES
MAMMALS
MAN
MATERIALS
MINUTES LIVING RADIOISOTOPES
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PRIMATES
PYRIDINES
QUINOLINES
RADIOISOTOPES
TISSUES
VERTEBRATES
560171* - Radiation Effects- Nuclide Kinetics & Toxicology- Man- (-1987)