Pretreatment with EDU decreases rat lung cellular responses to ozone

Journal Article · Tue Aug 01 00:00:00 EDT 1989 · Toxicology and Applied Pharmacology; (USA)

DOI:https://doi.org/10.1016/0041-008X(89)90089-6· OSTI ID:5471481

Bassett, D J; Elbon, C L; Reichenbaugh, S S; Boswell, G A; Stevens, T M; McGowan, M C; Kerr, J S ^[1]

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1. Johns Hopkins Univ., Baltimore, MD (USA)

The phenylurea compound EDU (N-(2-(2-oxo-1-imidazolindinyl)ethyl)-N'-phenylurea) has been shown to protect plants from the damaging effects of ozone exposure. Models of rat lung injury, based on acute exposure to 2 ppm ozone for 3 hr and on exposure to 0.85 ppm ozone for 2 days, were used to determine whether EDU pretreatment of rats protected lungs from oxidant injury. Rats were pretreated with 100 mg/kg body wt EDU by ip administration for 2 days prior to and on the days of ozone exposure. No adverse toxicological effects of EDU pretreatment were observed. Lung superoxide dismutase (SOD) and catalase (CAT) activities were significantly enhanced from 636 to 882 U/lung and from 599 to 856 U/lung, respectively. One day following acute exposure (2 ppm for 3 hr), an ozone-induced increase of polymorphonuclear leukocytes (PMNs) from 0.01 to 1.18 million cells/lung was decreased to 0.68 million by EDU pretreatment. No alteration occurred in the degree of lung permeability indicated by increased lavage fluid albumin. EDU pretreatment also significantly decreased ozone-induced increases in PMN recovery after 2 days exposure to 0.85 ppm ozone from 5.54 to 2.12 million cells/lung. However, in this second case, EDU pretreatment reduced the observed ozone damage, indicated by a decrease in lavage fluid albumin and by a decrease in the macrophage and lymphocyte infiltration associated with this length of ozone exposure. The observation that EDU-treated cultured pulmonary arterial endothelial cells increased SOD and CAT activities identified a potential lung site of EDU interaction. These data demonstrated that although EDU pretreatment appears not to prevent initial ozone damage, it does reduce the infiltration of PMNs and might therefore prevent amplification of the injury associated with this cell type.

• OSTI ID: 5471481
• Journal Information: Toxicology and Applied Pharmacology; (USA), Vol. 100:1; ISSN 0041-008X
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
LUNGS
PATHOLOGICAL CHANGES
OZONE
TOXICITY
UREA
BIOLOGICAL EFFECTS
CATALASE
ENZYME ACTIVITY
INTRAPERITONEAL INJECTION
LAVAGE
LEUKOCYTES
RATS
SUPEROXIDE DISMUTASE
WEIGHT
AMIDES
ANIMALS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
CARBONIC ACID DERIVATIVES
ENZYMES
INJECTION
INTAKE
MAMMALS
MATERIALS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
OXIDOREDUCTASES
PEROXIDASES
RESPIRATORY SYSTEM
RODENTS
VERTEBRATES
560300* - Chemicals Metabolism & Toxicology