Electron spin resonance study of free radicals produced from ethanol and acetaldehyde after exposure to a Fenton system or to brain and liver microsomes

Journal Article · Sun Sep 01 00:00:00 EDT 1991 · Alcohol; (United States)

DOI:https://doi.org/10.1016/0741-8329(91)90588-N· OSTI ID:5449061

Gonthier, B; Jeunet, A; Barret, L ^[1]

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1. Departement de Toxicologie, C.H.R.U. de Grenoble, (France)

Free radical formation from ethanol and acetaldehyde was studied in the presence of a spin-trap and a NADPH generating system with a chemical model, Fenton's reagent, or by enzymatic oxidation of these solvents by rat liver and brain microsomes. The free radicals were detected by electron spin resonance spectroscopy (E.S.R.), using the spin-trapping agent, alpha-(4-pyridyl l-oxide)-N-tertbutyl-nitrone (POBN). Under such conditions, the hydroxyethyl radical derived from ethanol was obtained after both incubation in liver and brain microsomes as well as after exposure to the Fenton system. Enzymatic inhibition and activation showed that the mixed function oxidase system plays an important role in the generation of such a radical, even in the brain. Under all the experimental conditions acetaldehyde could also generate a free radical deriving directly from the parent molecule and modified by enzymatic activation or inhibition. A second, longer lasting radical was also observed in the presence of acetaldehyde. On the basis of a comparative study to a known process causing lipoperoxidation, its lipidic origin was suggested.

• OSTI ID: 5449061
• Journal Information: Alcohol; (United States), Vol. 8:5; ISSN 0741-8329
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ACETALDEHYDE
METABOLISM
ETHANOL
RADICALS
BIOSYNTHESIS
BRAIN
ELECTRON SPIN RESONANCE
ENZYME ACTIVITY
HYDROGEN PEROXIDE
IRON
LIVER
MICROSOMES
MIXED-FUNCTION OXIDASES
NADP
RATS
REAGENTS
ALCOHOLS
ALDEHYDES
ANIMALS
BODY
CELL CONSTITUENTS
CENTRAL NERVOUS SYSTEM
COENZYMES
DIGESTIVE SYSTEM
ELEMENTS
ENZYMES
GLANDS
HYDROGEN COMPOUNDS
HYDROXY COMPOUNDS
MAGNETIC RESONANCE
MAMMALS
METALS
NERVOUS SYSTEM
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANS
OXIDOREDUCTASES
OXYGEN COMPOUNDS
OXYGENASES
PEROXIDES
PROTEINS
RESONANCE
RIBOSOMES
RODENTS
SYNTHESIS
TRANSITION ELEMENTS
VERTEBRATES
560300* - Chemicals Metabolism & Toxicology