Lymphocyte adhesion to endothelial cell (EC) is stimulated by phorbol esters
The effect of phorbol esters on T cell adhesion to EC has been studied. The phorbol esters 12-0-tetradecanoyl-phorbol-13-acetate and 4-beta-phorbol-12-13-dibutyrate, but not the biologically inert 4-0-methyl-phorbol-12-13-didecanoate strongly increased the binding of /sup 51/Cr-labeled T cells to human umbilical vein EC monolayers in microtiter wells. Increase in binding was observed at 0.3 ng/ml with maximal enhancement at 50 ng/ml. Both unstimulated and phorbol ester activated T cells displayed a substantially greater binding affinity for EC than for fibroblasts or plastic. Binding enhancement occurred within one minute, with maximal increase after 15 min. Preincubation studies showed that binding enhancement was entirely attributable to an effect on T cells, with no action on EC. Additive binding enhancement was seen when phorbol esters and reagents that increase adhesion by actions on EC (LPS, IL-1 and IFN-..gamma..) were used together. Increase in adhesion of activated T lymphocytes to EC may explain the greater emigration of activated T cells than small resting T cells into inflammatory foci in vivo. The rapid onset of the phorbol effect suggests that this may be an important mechanism for immediate localization of circulating T cells in the cellular immune response, activated, perhaps, at the endothelial blood-tissue interface.
- Research Organization:
- Univ. of Texas Health Science Center, Dallas
- OSTI ID:
- 5447585
- Report Number(s):
- CONF-8604222-
- Journal Information:
- Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 45:3; Conference: 70. annual meeting of the Federation of American Society for Experimental Biology, St. Louis, MO, USA, 13 Apr 1986
- Country of Publication:
- United States
- Language:
- English
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59 BASIC BIOLOGICAL SCIENCES
CHROMIUM
BIOLOGICAL EFFECTS
LYMPHOCYTES
ADHESION
PHORBOL ESTERS
BIOCHEMICAL REACTION KINETICS
ENDOTHELIUM
IMMUNE REACTIONS
TRACER TECHNIQUES
ANIMAL CELLS
ANIMAL TISSUES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
CARCINOGENS
CONNECTIVE TISSUE CELLS
ELEMENTS
ESTERS
ISOTOPE APPLICATIONS
KINETICS
LEUKOCYTES
MATERIALS
METALS
ORGANIC COMPOUNDS
REACTION KINETICS
SOMATIC CELLS
TISSUES
TRANSITION ELEMENTS
560301* - Chemicals Metabolism & Toxicology- Cells- (-1987)
551001 - Physiological Systems- Tracer Techniques