Intravascular streaming and variable delivery to brain following carotid artery infusions in the Sprague-Dawley rat

Journal Article · Mon Feb 01 00:00:00 EST 1988 · J. Cereb. Blood Flow Metab.; (United States)

DOI:https://doi.org/10.1038/jcbfm.1988.15· OSTI ID:5442390

Saris, S C; Wright, D C; Oldfield, E H; Blasberg, R G

Intracarotid artery infusions in animals are commonly performed in studies of the blood-brain barrier and in chemotherapy trials. Implicit in the analysis of these experiments is that the infusate will be distributed to the territory of the internal carotid artery in a manner that is proportional to blood flow. Fifteen Sprague-Dawley rats were studied to determine if poor infusate mixing with blood due to intravascular streaming occurred during intracarotid artery drug infusions and if it could be eliminated with fast retrograde infusion. In three experimental groups, a radiolabeled flow tracer--/sup 14/C-iodoantipyrine (IAP)--was infused retrograde through the external carotid artery into the common carotid artery at slow, medium, and fast rates (0.45, 1.5, and 5.0 ml/min). In a control group, IAP was injected intravenously (i.v.). Local isotope concentrations in the brain were determined by quantitative autoradiography, and the variability of isotope delivery was assessed in the frontoparietal cortex, temporal cortex, and caudate putamen of all animals. Streaming phenomena were manifest in all selected anatomic areas after the slow and medium rates of intraarterial infusion. After fast intracarotid infusion or i.v. injection, there was uniform distribution of isotope in the same brain regions.

• Research Organization: National Institute of Neurological and Communicative Disorders and Stroke, Bethesda, MD (USA)
• OSTI ID: 5442390
• Journal Information: J. Cereb. Blood Flow Metab.; (United States), Vol. 8:1
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
BRAIN
BLOOD FLOW
ANTIPYRINE
AUTORADIOGRAPHY
BLOOD-BRAIN BARRIER
CARBON 14 COMPOUNDS
CAROTID ARTERIES
INFUSION
INTRAVENOUS INJECTION
RATS
TISSUE DISTRIBUTION
ANIMALS
ANTIPYRETICS
ARTERIES
AZOLES
BLOOD VESSELS
BODY
CARDIOVASCULAR SYSTEM
CENTRAL NERVOUS SYSTEM
CENTRAL NERVOUS SYSTEM DEPRESSANTS
DISTRIBUTION
DRUGS
HETEROCYCLIC COMPOUNDS
INJECTION
INTAKE
LABELLED COMPOUNDS
MAMMALS
NERVOUS SYSTEM
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PYRAZOLES
PYRAZOLINES
RODENTS
VERTEBRATES
551001* - Physiological Systems- Tracer Techniques