Cloning and characterization of DNA complementary to the canine distemper virus mRNA encoding matrix, phosphoprotein, and nucleocapsid protein
Double-stranded cDNA synthesized from total polyadenylate-containing mRNA, extracted from monkey kidney cells infected with canine distemper virus (CDV), has been cloned into the PstI site of Escherichia coli plasmid pBR322. Clones containing canine distemper virus DNA were identified by hybridization to a canine distemper virus-specific, /sup 32/P-labeled cDNA. Four specific clones containing different classes of sequences have been identified. The cloned plasmids contain inserts of 800 (clone 44-80), 960 (clone 74-16), 1700 (clone 364), and 950 (clone 40-9) base pairs. The sizes of the mRNA species complementary to these inserts are 1500, 1850, 1850 and 2500 nucleotides, respectively, as determined by the Northern technique. Three of the cloned DNA fragments were further identified as the reverse transcripts of the mRNA coding for the matrix, phosphoprotein, and nucleocapsid protein of CDV.
- Research Organization:
- Weizmann Inst. of Science, Rehovot (Israel)
- OSTI ID:
- 5438673
- Journal Information:
- J. Virol.; (United States), Vol. 53:2
- Country of Publication:
- United States
- Language:
- English
Similar Records
Canine distemper virus neutralization activity is low in human serum and it is sensitive to an amino acid substitution in the hemagglutinin protein
The fusion protein of wild-type canine distemper virus is a major determinant of persistent infection
Related Subjects
VIRUSES
DNA SEQUENCING
DNA-CLONING
DNA
ESCHERICHIA COLI
GENETICS
HYBRIDIZATION
IN VITRO
KIDNEYS
LABELLED COMPOUNDS
MESSENGER-RNA
MONKEYS
NUCLEOTIDES
PHOSPHORUS 32
PLASMIDS
PROTEINS
TRACER TECHNIQUES
ANIMALS
BACTERIA
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGY
BODY
CELL CONSTITUENTS
CLONING
DAYS LIVING RADIOISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
MAMMALS
MICROORGANISMS
NUCLEI
NUCLEIC ACIDS
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANS
PARASITES
PHOSPHORUS ISOTOPES
PRIMATES
RADIOISOTOPES
RNA
STRUCTURAL CHEMICAL ANALYSIS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques
550401 - Genetics- Tracer Techniques