LPS-inducible factor(s) from activated macrophages mediates cytolysis of Naegleria fowleri amoebae
Soluble cytolytic factors of macrophage origin have previously been described with respect to their tumoricidal activity. The purpose of this study was to investigate the mechanism and possible factor(s) responsible for cytolysis of the amoeba Naegleria fowleri by activated peritoneal macrophages from B6C3F1 mice. Macrophages or conditioned medium (CM) from macrophage cultures were incubated with /sup 3/H-Uridine labeled amoebae. Percent specific release of label served as an index of cytolysis. Bacille Calmette-Guerin (BCG) and Corynebacterium parvum macrophages demonstrated significant cytolysis of amoebae at 24 h with an effector to target ratio of 10:1. Treatment of macrophages with inhibitors of RNA or protein synthesis blocked amoebicidal activity. Interposition of a 1 ..mu..m pore membrane between macrophages and amoebae inhibited killing. Inhibition in the presence of the membrane was overcome by stimulating the macrophages with LPS. CM from SPS-stimulated, but not unstimulated, cultures of activated macrophages was cytotoxic for amoebae. The activity was heat sensitive and was recovered from ammonium sulfate precipitation of the CM. Results indicate that amoebicidal activity is mediated by a protein(s) of macrophage origin induced by target cell contact or stimulation with LPS.
- Research Organization:
- Virginia Commonwealth Univ./MCV., Richmond
- OSTI ID:
- 5434028
- Report Number(s):
- CONF-8604222-; TRN: 86-026724
- Journal Information:
- Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 45:3; Conference: 70. annual meeting of the Federation of American Society for Experimental Biology, St. Louis, MO, USA, 13 Apr 1986
- Country of Publication:
- United States
- Language:
- English
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AMOEBA
LYSIS
LIPOPOLYSACCHARIDES
BIOLOGICAL EFFECTS
CELL MEMBRANES
MACROPHAGES
MEMBRANE PORES
MICE
NEOPLASMS
TRITIUM COMPOUNDS
TUMOR CELLS
URIDINE
ANIMAL CELLS
ANIMALS
AZINES
CARBOHYDRATES
CELL CONSTITUENTS
CONNECTIVE TISSUE CELLS
DISEASES
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
INVERTEBRATES
LABELLED COMPOUNDS
LIPIDS
MAMMALS
MEMBRANES
MICROORGANISMS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PHAGOCYTES
POLYSACCHARIDES
PROTOZOA
PYRIMIDINES
RIBOSIDES
RODENTS
SACCHARIDES
SARCODINA
SOMATIC CELLS
URACILS
VERTEBRATES
550901* - Pathology- Tracer Techniques