denV gene of bacteriophage T4 restores DNA excision repair to mei-9 and mus201 mutants of Drosophila melanogaster

Journal Article · Mon May 01 00:00:00 EDT 1989 · Proceedings of the National Academy of Sciences of the United States of America; (USA)

DOI:https://doi.org/10.1073/pnas.86.9.3227· OSTI ID:5430197

Banga, S S; Boyd, J B; Valerie, K; Harris, P V; Kurz, E M; de Riel, J K ^[1]

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1. Univ. of California, Davis (USA)

The denV gene of bacteriophage T4 was fused to a Drosophila hsp70 (70-kDa heat shock protein) promoter and introduced into the germ line of Drosophila by P-element-mediated transformation. The protein product of that gene (endonuclease V) was detected in extracts of heat-shocked transformants with both enzymological and immunoblotting procedures. That protein restores both excision repair and UV resistance to mei-9 and mus201 mutants of this organism. These results reveal that the denV gene can compensate for excision-repair defects in two very different eukayotic mutants, in that the mus201 mutants are typical of excision-deficient mutants in other organisms, whereas the mei-9 mutants exhibit a broad pleiotropism that includes a strong meiotic deficiency. This study permits an extension of the molecular analysis of DNA repair to the germ line of higher eukaryotes. It also provides a model system for future investigations of other well-characterized microbial repair genes on DNA damage in the germ line of this metazoan organism.

• OSTI ID: 5430197
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (USA), Vol. 86:9; ISSN 0027-8424
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
DROSOPHILA
DNA REPAIR
PROTEINS
BIOCHEMISTRY
PYRIMIDINE DIMERS
RADIOINDUCTION
BACTERIOPHAGES
BIOLOGICAL RADIATION EFFECTS
EXTREME ULTRAVIOLET RADIATION
MEIOSIS
PHOSPHORUS 32
ANIMALS
ARTHROPODS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
CELL DIVISION
CHEMISTRY
DAYS LIVING RADIOISOTOPES
DIPTERA
ELECTROMAGNETIC RADIATION
FLIES
FRUIT FLIES
INSECTS
INVERTEBRATES
ISOTOPES
LIGHT NUCLEI
MICROORGANISMS
NUCLEI
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PARASITES
PHOSPHORUS ISOTOPES
RADIATION EFFECTS
RADIATIONS
RADIOISOTOPES
RECOVERY
REPAIR
ULTRAVIOLET RADIATION
VIRUSES
560120* - Radiation Effects on Biochemicals
Cells
& Tissue Culture