Ethanol production by Saccharomyces cerevisiae immobilized in hollow-fiber membrane bioreactors
Saccharomyces cerevisiae ATCC 4126 was grown within the macroporous matrix of asymmetric-walled polysulfone hollow-fiber membranes and on the exterior surfaces of isotropic-walled polypropylene hollow-fiber membranes. Nutrients were supplied and products were removed by single-pass perfusion of the fiber lumens. Growth of yeast cells within the macrovoids of the asymmetric-walled membranes attained densities of greater than 10 to the power of 10 cells per ml and in some regions accounted for nearly 100% of the available macrovoid volume, forming a tissue-like mass. A radial distribution of cell packing existed across the fiber wall, indicating an inadequate glucose supply to cells located beyond 100 mum from the lumen surface. By comparison, yeast cell growth on the exterior surfaces of the isotropic-walled membranes resulted in an average density of 3.5 x 10 to the power of 9 viable cells per ml. Ethanol production by reactors containing isotropic polypropylene fibers reached a maximum value of 26 g/liter-h based on the total reactor volume. Reactor performance depended on the fiber packing density and on the glucose medium flow rate and was limited by low nutrient and product transport rates. The inhibition of ethanol production and the reduction in fermentation efficiency arose primarily from the accumulation of CO/sub 2/ gas within the sealed reactor shell space. (Refs. 37).
- Research Organization:
- Dept. of Chemical Engineering and Genetics, Stanford Univ., Stanford, CA 94305
- OSTI ID:
- 5424937
- Journal Information:
- Appl. Environ. Microbiol.; (United States), Vol. 46:1
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
ETHANOL
BIOSYNTHESIS
SACCHAROMYCES CEREVISIAE
IMMOBILIZED CELLS
BIOREACTORS
EFFICIENCY
FERMENTATION
INHIBITION
POLYPROPYLENE
ALCOHOLS
BIOCONVERSION
FUNGI
HYDROXY COMPOUNDS
MICROORGANISMS
ORGANIC COMPOUNDS
ORGANIC POLYMERS
PLANTS
POLYMERS
POLYOLEFINS
SACCHAROMYCES
SYNTHESIS
YEASTS
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