Inositol 1,4,5-trisphosphate-induced calcium release from platelet plasma membrane vesicles
A platelet membrane preparation, enriched in plasma membrane markers, took up /sup 45/Ca/sup 2 +/ in exchange for intravesicular Na+ and released it after the addition of inositol 1,4,5-trisphosphate (IP3). The possibility that contaminating dense tubular membrane (DTS) vesicles contributed the Ca/sup 2 +/ released by IP3 was eliminated by the addition of vanadate to inhibit Ca/sup +/-ATPase-mediated DTS Ca/sup 2 +/ sequestration and by the finding that only plasma membrane vesicles exhibit Na/sup +/-dependent Ca/sup 2 +/ uptake. Ca/sup 2 +/ released by IP3 was dependent on low extravesicular Ca/sup 2 +/ concentrations. IP3-induced Ca/sup 2 +/ release was additive to that released by Na/sup +/ addition while GTP or polyethylene glycol (PEG) had no effect. These results strongly suggest that IP3 facilitates extracellular Ca/sup 2 +/ influx in addition to release from DTS membranes.
- Research Organization:
- Univ. of Illinois, Chicago (USA)
- OSTI ID:
- 5410567
- Journal Information:
- Biochem. Biophys. Res. Commun.; (United States), Vol. 150:3
- Country of Publication:
- United States
- Language:
- English
Similar Records
Extracellular ATP-induced nuclear Ca{sup 2+} transient is mediated by inositol 1,4,5-trisphosphate receptors in mouse pancreatic {beta}-cells
Formation and actions of calcium-mobilizing messenger, inositol 1,4,5-trisphosphate
Related Subjects
CALCIUM
MEMBRANE TRANSPORT
PHOSPHOLIPIDS
BIOLOGICAL EFFECTS
BLOOD PLATELETS
CALCIUM 45
CELL MEMBRANES
KINETICS
POLYETHYLENE GLYCOLS
SODIUM
TRACER TECHNIQUES
VANADATES
ALCOHOLS
ALKALI METALS
ALKALINE EARTH ISOTOPES
ALKALINE EARTH METALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CALCIUM ISOTOPES
CELL CONSTITUENTS
DAYS LIVING RADIOISOTOPES
ELEMENTS
ESTERS
EVEN-ODD NUCLEI
GLYCOLS
HYDROXY COMPOUNDS
INTERMEDIATE MASS NUCLEI
ISOTOPE APPLICATIONS
ISOTOPES
LIPIDS
MATERIALS
MEMBRANES
METALS
NUCLEI
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
ORGANIC POLYMERS
OXYGEN COMPOUNDS
POLYMERS
RADIOISOTOPES
TRANSITION ELEMENT COMPOUNDS
VANADIUM COMPOUNDS
550201* - Biochemistry- Tracer Techniques