Molecular cloning and characterization of a mammalian excision repair gene that partially restores UV resistance to xeroderma pigmentosum complementation group D cells
- Univ. of Cambridge (England)
A hamster DNA repair gene has been isolated by cosmid rescue after two rounds of transfection of an immortalized xeroderma pigmentosum (XP) complementation group D cell line with neomycin-resistance gene (neo)-tagged normal hamster DNA and selection with G418 and ultraviolet irradiation. The functional length of the sequence has been defined as 11.5 kilobase pairs by measurement of the region of overlap between two hamster DNA-containing cosmids, cloned by selection for the integrated neo gene, that are able to confer an increase in resistance to ultraviolet irradiation on two XP-D cell line but not on an XP-A line. Detailed molecular characterization of the hamster repair gene has revealed no obvious similarities to two human excision repair genes (ERCC1 and ERCC2) that correct repair-defective hamster cells but have no effect on XP cells. Hybridization analyses of normal human and XP cell genomic DNAs and mRNAs, using a cosmid-clone probe from which repeated sequences have been removed, show that homologues are present and expressed in all cases.
- OSTI ID:
- 5407022
- Journal Information:
- Proceedings of the National Academy of Sciences of the United States of America; (USA), Vol. 86:18; ISSN 0027-8424
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
EXCISION REPAIR
GENES
XP CELLS
RADIOSENSITIVITY
CELL CULTURES
DNA
DNA HYBRIDIZATION
DNA-CLONING
HAMSTERS
MAN
ULTRAVIOLET RADIATION
XERODERMA PIGMENTOSUM
ANIMAL CELLS
ANIMALS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
CLONING
DISEASES
DNA REPAIR
ELECTROMAGNETIC RADIATION
HYBRIDIZATION
MAMMALS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PRIMATES
RADIATIONS
RECOVERY
REPAIR
RODENTS
SKIN DISEASES
VERTEBRATES
560120* - Radiation Effects on Biochemicals
Cells
& Tissue Culture