An improved method of electroporation for introducing biologically active foreign genes into cultured mammalian cells

Journal Article · Thu Sep 01 00:00:00 EDT 1988 · Experimental Cell Research; (United States)

DOI:https://doi.org/10.1016/0014-4827(88)90386-2· OSTI ID:5393224

Tatsuka, Masaaki; Orita, Satoshi; Yagi, Takashi; Kakunaga, Takeo ^[1]

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1. Osaka Univ. (Japan)

The authors have developed a modified, reproducible, and efficient method for introducing cloned genes into mammalian cells by using an electric field followed by treatment with sodium butyrate. Transfection frequencies with plasmid pSV2-neo, consisting of an antibiotic (G418) resistance gene and simian virus 40 (SV40) early promoter, by electroporation were higher than those by calcium phosphate DNA precipitation. Treatment with sodium butyrate following electroporation significantly increased the frequency of transfection in various types of cell lines and primary cultured cells including human skin fibroblasts. Treatment with sodium butyrate also increased the transient expression of the gene for chloramphenicol acetyltransferase when the gene was introduced into BALB/c 3T3 cells by eletroporation. Electroporation combined with sodium butyrate treatment is an improved method for stable and transient biochemical transformation of foreign genes in cultured mammalian cells.

• OSTI ID: 5393224
• Journal Information: Experimental Cell Research; (United States), Vol. 178:1; ISSN 0014-4827
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
ELECTRIC FIELDS
BIOLOGICAL EFFECTS
FIBROBLASTS
GENE RECOMBINATION
GENES
DNA-CLONING
BUTYRIC ACID
MAN
MICE
RATS
TRANSFERASES
ANIMAL CELLS
ANIMALS
CARBOXYLIC ACIDS
CLONING
CONNECTIVE TISSUE CELLS
DNA HYBRIDIZATION
ENZYMES
HYBRIDIZATION
MAMMALS
MONOCARBOXYLIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
PRIMATES
RODENTS
SOMATIC CELLS
VERTEBRATES
550400* - Genetics