The repair of full-thickness articular cartilage defects. Immune responses to reparative tissue formed by allogeneic growth plate chondrocyte implants
- Department of Orthopaedics, Shimane Medical School, Izumo (Japan)
Growth plate cartilage cultivated in vitro was attached with a fibrin clot to a full-thickness articular cartilage defect on knee joints in allogeneic New Zealand rabbits. The healing of the defects was assessed by gross examination, light microscopy, and immunologic analysis for 24 weeks. Immunologic assessment of cell-mediated immunity, cytotoxicity of a humoral antibody by a 51 chromium release assay, and immunofluorescence studies were carried out. During the first two weeks following grafting, healing was excellent in 11 of the 17 defects. From three to 24 weeks, 11 of 42 defects examined had good results. Host lymphocytes had accumulated around the allograft at two to 12 weeks. Most of the implanted cartilage grown in vitro died and was replaced by fibrous tissue. The immunologic studies suggested that the implanted cartilage began to degenerate two to three weeks after implantation partially because of a humoral immune response but more importantly because of cell-mediated cytotoxicity.
- OSTI ID:
- 5390914
- Journal Information:
- Clinical Orthopaedics and Related Research; (United States), Vol. 268; ISSN 0009-921X
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
CARTILAGE
BIOLOGICAL REPAIR
WOUNDS
HEALING
BONE JOINTS
CHROMIUM 51
FIBRIN
FLUORESCENCE
GRAFTS
IMMUNE REACTIONS
LEGS
LYMPHOCYTES
RABBITS
TISSUE CULTURES
TRACER TECHNIQUES
ANIMAL CELLS
ANIMAL TISSUES
ANIMALS
BETA DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BIOLOGICAL RECOVERY
BLOOD
BLOOD CELLS
BLOOD COAGULATION FACTORS
BODY
BODY AREAS
BODY FLUIDS
CHROMIUM ISOTOPES
COAGULANTS
CONNECTIVE TISSUE
CONNECTIVE TISSUE CELLS
DAYS LIVING RADIOISOTOPES
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
EVEN-ODD NUCLEI
HEMATOLOGIC AGENTS
HEMOSTATICS
INJURIES
INTERMEDIATE MASS NUCLEI
ISOTOPE APPLICATIONS
ISOTOPES
LEUKOCYTES
LIMBS
LUMINESCENCE
MAMMALS
MATERIALS
NUCLEI
ORGANIC COMPOUNDS
ORGANS
PROTEINS
RADIOISOTOPES
RECOVERY
REPAIR
SCLEROPROTEINS
SKELETON
SOMATIC CELLS
TISSUES
TRANSPLANTS
VERTEBRATES
550901* - Pathology- Tracer Techniques