Concentration of butadiene diepoxide in rat tissues following low-level and high-level inhalation exposures to 1,3-butadiene
- Inhalation Toxicology Research Inst., Albuquerque, NM (United States); and others
1,3-Butadiene (BD), a chemical used extensively in the rubber industry, is carcinogenic in mice following chronic exposures as low as 6.25 ppm, but is carcinogenic in rats only after exposures of 1000 and 8000 ppm. Previous studies in our laboratory have shown that levels of the highly mutagenic metabolite, butadiene diepoxide (BDO{sub 2}), are much greater in mouse tissues than rat tissues after exposures of 62.5 ppm BD. We hypothesized that this metabolite is the primary determinant of BD carcinogenicity and hence would increase in rats following high-level exposures to BD. Female Sprague-Dawley rats were exposed to 62.5 ppm BD (low-level) and 8000 ppm BD (high-level) for either 6 h or 6 h daily for 10 days. Blood was collected immediately following the exposure and analyzed for the presence of BDO{sub 2} by multidimensional gas chromatography/mass spectrometry. Following exposures of 6 h or 6 h/day for 10 days to 62.5 ppm BD, levels of BDO{sub 2} in rat blood were 14 {+-} 2 (mean {+-} SE) and 17 {+-} 2 pmol/g, respectively. Levels of BDO{sub 2} in mouse blood following identical exposures were 15- to 25-fold greater than those observed in rat blood. Following exposures of rats to 8000 ppm BD, blood levels of BDO{sub 2} were very similar to those observed after exposures to 62.5 ppm RD (11 {+-} 1 and 17{+-}1 pmol/g following exposures of 6 h and 6 h/day for 10 days, respectively). This study shows that levels of BDO{sub 2}, do not increase in the rat following a high-level exposure to BD, suggesting that factors other than the production of this metabolite may be involved in BD-induced tumors in rats.
- OSTI ID:
- 538961
- Report Number(s):
- CONF-960807-; TRN: 97:004029-0028
- Resource Relation:
- Conference: 212. national meeting of the American Chemical Society (ACS), Orlando, FL (United States), 25-30 Aug 1996; Other Information: PBD: 1996; Related Information: Is Part Of 212th ACS national meeting; PB: 1830 p.
- Country of Publication:
- United States
- Language:
- English
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