Identification of an alpha sub 2 -adrenoceptor in human coronary arteries by radioligand binding assay

Journal Article · Tue Jan 01 00:00:00 EST 1991 · Life Sciences; (United States)

DOI:https://doi.org/10.1016/0024-3205(91)90606-C· OSTI ID:5381842

Ishikawa, Y; Umemura, S; Uchino, K; Shindou, T; Yasuda, G; Minamisawa, K; Hayashi, S; Hirawa, N; Ishii, M ^[1]

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1. Yokohama City Univ. (JAPAN)

A single high affinity binding site for an alpha{sub 2}-adrenoceptor in human coronary arteries was identified by radioligand binding assay. Human coronary arteries were obtained at autopsy within 6 hours of death. A crude membrane solution was incubated with ({sup 3}H)-rauwolscine at 25C for 30 min. The binding of ({sup 3}H)-rauwolscine was rapidly saturable and reversible. Kd was 1.2 {plus minus} 0.2 (SE) nM and Bmax 22 {plus minus} 3 fmol/mg protein. This is the first study which has shown the presence of an alpha{sub 2}-adrenoceptor in human coronary arteries using a radioligand binding assay method.

• OSTI ID: 5381842
• Journal Information: Life Sciences; (United States), Vol. 48:26; ISSN 0024-3205
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
RECEPTORS
RADIOASSAY
SYMPATHOMIMETICS
AFFINITY
BIOCHEMICAL REACTION KINETICS
CELL MEMBRANES
CORONARIES
MAN
TRACER TECHNIQUES
TRITIUM COMPOUNDS
ANIMALS
ARTERIES
AUTONOMIC NERVOUS SYSTEM AGENTS
BLOOD VESSELS
BODY
CARDIOVASCULAR SYSTEM
CELL CONSTITUENTS
DRUGS
HYDROGEN COMPOUNDS
ISOTOPE APPLICATIONS
KINETICS
MAMMALS
MEMBRANE PROTEINS
MEMBRANES
ORGANIC COMPOUNDS
ORGANS
PRIMATES
PROTEINS
REACTION KINETICS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques