Cadmium-binding protein (metallothionein) in carp
When carp (Cyprinus carpio) were exposed to 5 and 30 ppm Cd in the water, the contents of Cd-binding protein, which has low molecular weight, increased in the hepatopancreas, kidney, gills and gastrointestinal tract with duration of exposure. This Cd-binding protein was purified from hepatopancreas, kidney, gills, and spleen of carp administered 2 mg/kg Cd (as CdCl/sub 2/), intraperitoneally for 6 days. Two Cd-binding proteins were separated by DEAE-Sephadex A-25 column chromatography. These proteins had Cd-mercaptide bond, high cysteine contents (ca. 29-34%), but no aromatic amino acids or histidine. From these characteristics the Cd-binding proteins were identified as metallothionein. By using antiserum obtained from a rabbit to which carp hepatopancreas MT-II had been administered, immunological characteristics between hepatopancreas MT-I, II and kidney MT-II were studied, and a slight difference in antigenic determinant was observed among them. By immunological staining techniques with horseradish peroxidase, the localization of metallothionein was investigated. Carp were bred in 1 ppm Cd, 5 ppm Zn solution, and tap water for 14 days, following transfer to 15 ppm Cd solution, respectively. The survival ratio was the highest in the Zn group followed by Cd-treated and control groups.
- Research Organization:
- Gifu Pharmaceutical Univ., Japan
- OSTI ID:
- 5381782
- Journal Information:
- Environ. Health Perspect.; (United States), Vol. 65
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
CADMIUM CHLORIDES
BIOLOGICAL EFFECTS
METALLOTHIONEIN
BIOCHEMISTRY
TISSUE DISTRIBUTION
FISHES
GASTROINTESTINAL TRACT
GILLS
ION EXCHANGE CHROMATOGRAPHY
KIDNEYS
LIVER
PANCREAS
SPLEEN
ANIMALS
AQUATIC ORGANISMS
BODY
CADMIUM COMPOUNDS
CADMIUM HALIDES
CHEMISTRY
CHLORIDES
CHLORINE COMPOUNDS
CHROMATOGRAPHY
DIGESTIVE SYSTEM
DISTRIBUTION
ENDOCRINE GLANDS
GLANDS
HALIDES
HALOGEN COMPOUNDS
METALLOPROTEINS
ORGANIC COMPOUNDS
ORGANS
PROTEINS
RESPIRATORY SYSTEM
SEPARATION PROCESSES
VERTEBRATES
560305* - Chemicals Metabolism & Toxicology- Vertebrates- (-1987)
550200 - Biochemistry