Kinetic investigation of the catalytic mechanism for bovine liver mitochondrial monoamine oxidase
The kinetic behavior of the oxidative deamination reaction catalyzed by bovine liver mitochondrial monoamine oxidase was investigated with a series of ring-substituted benzylamines. Oxidation rates were fastest with the meta isomers. Dalziel coefficients were consistent with a mechanism involving a ternary complex for all substrates tested. Alterations in the Michaelis constant for oxygen were similar in magnitude to those for the rate of catalysis. Deuterium and tritium isotope effects were determined to obtain more detailed information on the mechanism of catalysis. Large deuterium isotope effects expressed on k{sub cat} were obtained for all substrates. Determination of the tritium isotope effect for benzylamine allowed the calculation of an intrinsic isotope effect of 6.5 and a secondary isotope effect of 1.17. Steady-state experiments were supplemented with pre-steady-state kinetic techniques. Rates of flavin reduction were faster than that of turnover. The deuterium isotope effect obtained for the rate of flavin reduction was 7-15 for the various substrates. The observed isotope effect was found to be an appropriate estimate for the intrinsic isotope effect.
- Research Organization:
- Emory Univ., Atlanta, GA (USA)
- OSTI ID:
- 5363051
- Resource Relation:
- Other Information: Thesis (Ph. D.)
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
AMINE OXIDASES
BIOCHEMICAL REACTION KINETICS
DEUTERIUM
ISOTOPE EFFECTS
FLAVINES
METABOLISM
OXIDOREDUCTASES
TRITIUM COMPOUNDS
CATALYSIS
CATTLE
DEAMINATION
LIVER
MITOCHONDRIA
STEADY-STATE CONDITIONS
SUBSTRATES
TRACER TECHNIQUES
ACRIDINES
AMINES
ANIMALS
AROMATICS
AZAARENES
AZINES
BODY
CELL CONSTITUENTS
CHEMICAL REACTIONS
DIGESTIVE SYSTEM
DOMESTIC ANIMALS
ENZYMES
GLANDS
HETEROCYCLIC COMPOUNDS
HYDROGEN COMPOUNDS
HYDROGEN ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LIGHT NUCLEI
MAMMALS
NUCLEI
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANOIDS
ORGANS
PYRIDINES
REACTION KINETICS
RUMINANTS
STABLE ISOTOPES
VERTEBRATES
550501* - Metabolism- Tracer Techniques