Bovine parathyroid hormone-(41-84), a hormone fragment with desirable properties for use as radioligand

Journal Article · Tue Dec 01 00:00:00 EST 1981 · J. Lab. Clin. Med.; (United States)

OSTI ID:5346722

Mallette, L E; Bradley, W A

Radioiodinated bPTH has been widely used as the labeled ligand in the radioimmunoassay of PTH. We now report the properties of a carboxyterminal fragment of bPTH that has several favorable characteristics when used as radioligand. This peptide, the chief component of a commercial preparation of bPTH, was isolated by gel filtration, where it migrated more slowly than did authentic bPTH-(1-84). It yielded lower nonspecific binding values and more sensitive hPTH assays than were seen with the intact hormone. By immunological criteria this peptide lacked the aminoterminal region of PTH, since hPTH-(1-34) did not inhibit its binding to any of 11 different antisera with known ability to recognize the aminoterminal region of PTH. The peptide did not contain most or all of the carboxyterminal region, however, since its binding to anti-PTH sera was inhibited by hPTH-(44-68) or hPTH-(53-84). Sequential Edman degradation of the iodinated peptide released iodotyrosine at the third cycle, suggesting the structure, bPTH-(41-84). The lower nonspecific binding and enhanced assay sensitivity provided by this peptide suggest that the use of other natural or synthetic fragments of PTH as radioligands might enhance the performance of PTH assays.

• Research Organization: Medical and Research Services, Veterans Administration Medical Center, Houston, Texas
• OSTI ID: 5346722
• Journal Information: J. Lab. Clin. Med.; (United States), Vol. 98:6
• Country of Publication: United States
• Language: English

Related Subjects

62 RADIOLOGY AND NUCLEAR MEDICINE
PARATHORMONE
RADIOIMMUNOASSAY
CATTLE
CHEMICAL BONDS
IMMUNE SERUMS
IODINE COMPOUNDS
LABELLED COMPOUNDS
PEPTIDES
ANIMALS
DOMESTIC ANIMALS
HALOGEN COMPOUNDS
HORMONES
ISOTOPE APPLICATIONS
MAMMALS
ORGANIC COMPOUNDS
PEPTIDE HORMONES
PROTEINS
RADIOASSAY
RUMINANTS
TRACER TECHNIQUES
VERTEBRATES
550601* - Medicine- Unsealed Radionuclides in Diagnostics