Immunolocalization of 7-2-ribonucleoprotein in the granular component of the nucleolus

Journal Article · Sun May 01 00:00:00 EDT 1988 · Experimental Cell Research; (United States)

DOI:https://doi.org/10.1016/0014-4827(88)90126-7· OSTI ID:5322326

Reimer, G ^[1]; Raska, I ^[2]; Scheer, U ^[3]; Tan, E M ^[4]

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1. Univ. of Erlangen-Nuernberg (West Germany)
2. Institute of Experimental Medicine, Prague (Czechoslovakia)
3. Univ. of Wuerzburg (West Germany)
4. Scripps Clinic and Research Foundation, La Jolla, CA (USA)

Certain autoimmune sera contain antibodies against a nucleolar ribonucleotprotein particle associated with 7-2-RNA. In this study, the authors showed by immunofluorescence microscopy that antibodies reactive with 7-2-ribonucleoprotein immunolocalized in the granular regions of actinomycin D and 5,6-dichloro-1-{beta}-D-ribofuranosylbenzimidazole (DRB)--segregated nucleoli from Vero cells. By electron microscopic immunocytochemistry, antigen-antibody complexes were located in the granular component of transcriptionally active nucleoli from rat liver hepatocytes and HeLa cells. Anti-7-2-RNP antibodies from two autoimmune sera immunoprecipitated a major protein of M{sub r} 40,000 from ({sup 35}S) methionine-labeled HeLa cell extract. The immunolocalization data suggest that 7-2-ribonucleoprotein may be involved in stages of ribosome biogenesis which take place in the granular component of the nucleolus, i.e., assembly, maturation, and/or transport of preribosomes.

• OSTI ID: 5322326
• Journal Information: Experimental Cell Research; (United States), Vol. 175:2; ISSN 0014-4827
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
NUCLEOPROTEINS
SUBCELLULAR DISTRIBUTION
RIBOSOMES
BIOSYNTHESIS
CYTOCHEMISTRY
FLUORESCENCE
HELA CELLS
IMIDAZOLES
IMMUNE SERUMS
IMMUNOASSAY
LIVER CELLS
METHIONINE
MICROSCOPY
PHOSPHORUS 32
RATS
SULFUR 35
AMINO ACIDS
ANIMAL CELLS
ANIMALS
AZOLES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOASSAY
BIOCHEMISTRY
CARBOXYLIC ACIDS
CELL CONSTITUENTS
CHEMISTRY
DAYS LIVING RADIOISOTOPES
DISTRIBUTION
DRUGS
EVEN-ODD NUCLEI
HETEROCYCLIC COMPOUNDS
ISOTOPES
LIGHT NUCLEI
LIPOTROPIC FACTORS
LUMINESCENCE
MAMMALS
NUCLEI
ODD-ODD NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PHOSPHORUS ISOTOPES
PROTEINS
RADIOISOTOPES
RODENTS
SOMATIC CELLS
SULFUR ISOTOPES
SYNTHESIS
VERTEBRATES
550301* - Cytology- Tracer Techniques