Phorbol esters enhance attachment of NIH/3T3 cells to laminin and type IV collagen substrates

Journal Article · Tue Nov 01 00:00:00 EST 1988 · Experimental Cell Research; (United States)

DOI:https://doi.org/10.1016/0014-4827(88)90345-X· OSTI ID:5317383

Kato, Shigemi; Ben, T L; De Luca, L M ^[1]

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1. National Institutes of Health, Bethesda, MD (USA)

The effect of phorbol esters on the adhesive properties of NIH/3T3 mouse fibroblasts was investigated using plastic substrates precoated with the extracellular matrix proteins fibronectin, collagen, and laminin. Treatment with phorbol 12-myristate 13-acetate (PMA) enhanced NIH/3T3 cell attachment to laminin and type IV collagen substrates but had little or no effect on attachment to fibronectin and type I collagen substrates. The effect of PMA in enhancing cell attachment to laminin and type IV collagen substrates was dose dependent between 10{sup {minus}9} and 10{sup {minus}7} M. PMA was effective as early as 30 min; the effect reached a maximum at 2 h and decreased gradually. Phorbol 12, 13-dibenzoate and phorbol 12, 13-diacetate were effective but to a lesser extent and phorbol 12-myristate and phorbol 13-acetate showed little or no effect. These results suggest that PMA may enhance NIH/3T3 cell adhesion through effects on laminin and type IV collagen receptors. Retinoic acid, which itself requires at least 6 h to show an effect on attachment, did not have any effect on cell attachment in 2 h and, if anything, slightly inhibited PMA-enhanced cell attachment to laminin and type IV collagen substrates.

• OSTI ID: 5317383
• Journal Information: Experimental Cell Research; (United States), Vol. 179:1; ISSN 0014-4827
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
FIBROBLASTS
ADHESION
PHORBOL ESTERS
BIOLOGICAL EFFECTS
SCLEROPROTEINS
BIOCHEMISTRY
COLLAGEN
MORPHOLOGY
RECEPTORS
RETINOIC ACID
ANIMAL CELLS
CARBOXYLIC ACID ESTERS
CARCINOGENS
CHEMISTRY
CONNECTIVE TISSUE CELLS
ESTERS
MEMBRANE PROTEINS
ORGANIC COMPOUNDS
PROTEINS
SOMATIC CELLS
560300* - Chemicals Metabolism & Toxicology