Heat-induced accumulation and futile cycling of trehalose in Saccharomyces cerevisiae

Journal Article · Tue Dec 01 00:00:00 EST 1987 · J. Bacteriol.; (United States)

OSTI ID:5316875

Hottiger, T; Schmutz, P; Wiemken, A

Heat shock resulted in rapid accumulation of large amounts of trehalose in Saccharomyces cerevisiae. In cultures growing exponentially on glucose, the trehalose content of the cells increased from 0.01 to 1 g/g of protein within 1 h after the incubation temperature was shifted from 27 to 40/sup 0/C. When the temperature was readjusted to 27/sup 0/C, the accumulated trehalose was rapidly degraded. In parallel, the activity of the trehalose-phosphate synthase, the key enzyme of trehalose biosynthesis, increased about six fold during the heat shock and declined to normal level after readjustment of the temperature. Surprisingly, the activity of neutral trehalase, the key enzyme of trehalose degradation, also increased about threefold during the heat shock and remained almost constant during recovery of the cells at 27/sup 0/C. In pulse-labeling experiments with (/sup 14/C) glucose, trehalose was found to be turned over rapidly in heat-shocked cells, indicating that both anabolic and catabolic enzymes of trehalose metabolism were active in vivo. Possible functions of the heat-induced accumulation of trehalose and its rapid turnover in an apparently futile cycle during heat shock are discussed.

• Research Organization: Univ. of Basel, Switzerland
• OSTI ID: 5316875
• Journal Information: J. Bacteriol.; (United States), Vol. 169:12
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
DISACCHARIDES
BIOLOGICAL ACCUMULATION
METABOLISM
SACCHAROMYCES CEREVISIAE
TEMPERATURE EFFECTS
CARBON 14 COMPOUNDS
ENZYME ACTIVITY
TRACER TECHNIQUES
CARBOHYDRATES
FUNGI
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MICROORGANISMS
OLIGOSACCHARIDES
ORGANIC COMPOUNDS
PLANTS
SACCHARIDES
SACCHAROMYCES
YEASTS
550201* - Biochemistry- Tracer Techniques