Demonstration of activation of B lymphocytes in New Zealand black mice at birth by an immunoradiometric assay for murine IgM

Journal Article · Tue Nov 01 00:00:00 EST 1977 · J. Immunol.; (United States)

OSTI ID:5301413

Moutsopoulos, H M; Boehm-Truitt, M; Kassan, S S; Chused, T M

We have developed a highly specific and sensitive two-site immunoradiometric assay for murine IgM in which antigen bound to immobilized antibody reacts with affinity-purified radiolabeled antibody. We utilized the sensitivity of this assay to study the rate of IgM secretion in short-term cultures by spleen cells from the autoimmune strains, New Zealand Black (NZB) and New Zealand Black by New Zealand White F/sub 1/ hybrid (BW), and from normal (C57BL/6, DBA/2, NZW) mice. The temperature dependence of IgM secretion in short-term cultures, its pentameric structure, and the similar viability of NZB and normal strain spleen cells indicate that active IgM synthesis is being measured. We observed that the splenic B lymphocytes of NZB and BW mice, in contrast to normal strains, produce IgM in vitro at birth. By 6 to 8 weeks of age NZB and BW spleen cells produce 40 times more IgM than spleen cells from normal strains of mice. The IgM produced in vitro by splenic lymphocytes from NZB and BW mice is not absorbed by syngeneic or allogeneic thymocytes or erythrocytes.

• Research Organization: National Inst. of Dental Research, Bethesda, MD
• OSTI ID: 5301413
• Journal Information: J. Immunol.; (United States), Vol. 119:5
• Country of Publication: United States
• Language: English

Related Subjects

62 RADIOLOGY AND NUCLEAR MEDICINE
IMMUNOGLOBULINS
RADIOIMMUNOASSAY
LYMPHOCYTES
CHEMICAL ACTIVATION
ANTIBODIES
ANTIGENS
MICE
RADIOISOTOPES
SPLEEN CELLS
TRACER TECHNIQUES
ANIMAL CELLS
ANIMALS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CONNECTIVE TISSUE CELLS
GLOBULINS
ISOTOPE APPLICATIONS
ISOTOPES
LEUKOCYTES
MAMMALS
ORGANIC COMPOUNDS
PROTEINS
RADIOASSAY
RODENTS
SOMATIC CELLS
VERTEBRATES
550601* - Medicine- Unsealed Radionuclides in Diagnostics