Ultraviolet-induced reversion of cyc1 alleles in radiation-sensitive strains of yeast. III. rev3 mutant strains
The role of the REV3 gene function in uv-induced mutagenesis in the yeast Saccharomyces cerevisiae has been examined by determining the reversion of 12 well-defined cyc1 mutations in diploid strains homozygous for the rev3-1 or rev3-3 allele. The 12 cyc1 alleles include one ochre, one amber, four initiation, two proline missense, and four frameshift mutations. We find that the rev3 mutations reduce the frequency of uv-induced reversion of all of the cyc1 alleles, though different classes of alleles respond to a different extent. These results imply that the REV3 gene function is required for the production of a wide variety of mutational events, though probably not all, and show that each of the three REV loci have different mutational phenotypes. Such diverse phenotypes are not predicted by the unitary model for bacterial mutagenesis, suggesting that this is at best an imcomplete description of eukaryotic mutagenesis.
- Research Organization:
- Univ. of Rochester, NY
- OSTI ID:
- 5298934
- Journal Information:
- Genetics; (United States), Vol. 92
- Country of Publication:
- United States
- Language:
- English
Similar Records
Ultraviolet-induced reversion of cyc1 alleles in radiation sensitive strains of yeast. II. rev2 mutant strains
UV mutagenesis in radiation-sensitive strains of yeast
Related Subjects
59 BASIC BIOLOGICAL SCIENCES
MUTAGENESIS
RADIOINDUCTION
SACCHAROMYCES CEREVISIAE
RADIOSENSITIVITY
ULTRAVIOLET RADIATION
GENETIC RADIATION EFFECTS
EXPERIMENTAL DATA
GENES
GENOME MUTATIONS
ISOLATED VALUES
MUTATION FREQUENCY
NUCLEOTIDES
PHENOTYPE
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
DATA
DATA FORMS
ELECTROMAGNETIC RADIATION
FUNGI
GENETIC EFFECTS
INFORMATION
MICROORGANISMS
MUTATIONS
NUMERICAL DATA
ORGANIC COMPOUNDS
PLANTS
RADIATION EFFECTS
RADIATIONS
SACCHAROMYCES
YEASTS
560131* - Radiation Effects on Microorganisms- Basic Studies- (-1987)
550400 - Genetics
550200 - Biochemistry