Laboratory methods for determining the effects of bactericides on acid mine drainage

Conference · Sat Dec 01 00:00:00 EST 1984 · Univ. Ky., Off. Eng. Serv., (Bull.); (United States)

OSTI ID:5288573

Shellhorn, M A; Rastogi, V

Chemolithotrophic bacteria, particularly Thiobacillus ferrooxidans, can accelerate the oxidation of iron sulfide minerals. Materials toxic to these bacteria can be used to control acid mine drainage. For practical application, it is necessary to evaluate bactericides by determining the concentration at which they are effective for the spoil or refuse on which they are to be used. Two laboratory methods have been developed. In the batch method, ten-gram aliquots of ground refuse powder treated with water or bactericide solutions were incubated in centrifuge bottles for specified periods of time in a humidity chamber. Analyses were done by extracting the samples into a measured volume of water and assaying. In the column leach method, glass columns were used containing one-kilogram samples of refuse of controlled particle size distribution to ensure uniform flow characteristics in all columns. A system for metering water or bactericide solutions into the columns at pre-determined rates was used. Column effluents were assayed weekly. In both methods, comparisons with controls were used to determine the effects of bactericide treatments. Test protocol, versatility and limitations are described using sodium lauryl sulfate as the bactericide.

• Research Organization: BF Goodrich Co., Brecksville, OH
• OSTI ID: 5288573
• Report Number(s): CONF-841204-
• Journal Information: Univ. Ky., Off. Eng. Serv., (Bull.); (United States), Conference: National symposium on surface mining, hydrology, sedimentology and reclamation, Lexington, KY, USA, 2 Dec 1984
• Country of Publication: United States
• Language: English

Related Subjects

01 COAL, LIGNITE, AND PEAT
54 ENVIRONMENTAL SCIENCES
ACID MINE DRAINAGE
WATER POLLUTION CONTROL
SURFACE MINING
BENCH-SCALE EXPERIMENTS
GERMICIDES
COAL MINING
EXPERIMENT PLANNING
IRON SULFIDES
OXIDATION
SPOIL BANKS
THIOBACILLUS FERROXIDANS
WATER POLLUTION
BACILLUS
BACTERIA
CHALCOGENIDES
CHEMICAL REACTIONS
CONTROL
IRON COMPOUNDS
MICROORGANISMS
MINING
PLANNING
POLLUTION
POLLUTION CONTROL
SULFIDES
SULFUR COMPOUNDS
SULFUR-OXIDIZING BACTERIA
TRANSITION ELEMENT COMPOUNDS
010800* - Coal
Lignite
& Peat- Waste Management
520200 - Environment
Aquatic- Chemicals Monitoring & Transport- (-1989)