Studies of endogenous UDP-glucose: glycoprotein glucose-1-phosphotransferase acceptors in embryonic chick neural tissues

Thesis/Dissertation · Wed Jan 01 00:00:00 EST 1986

OSTI ID:5281594

Hiller, A M.S.

UPD-glucose: glycoprotein glucose-1-phosphotransferase (GlcPTase) catalyzes the transfer of a Glc-1-P from UDP-Glc to high mannose-type oligosaccharides on a class of embryonic chick neutral retinal glycoproteins. The primary acceptors for this glycosylphosphotransferase were determined by incubating embryonic chick tissue sonicates with (B-/sup 32/P)UDP-Glc followed by SDS-polyacrylamine gel electrophoresis and autoradiography. The primary GlcPT ase acceptors in brain tissue have apparent subunit molecular weights of 66 and 62 kilodaltons (kd). /sup 32/P incorporation into these peptides was eliminated by excess levels of unlabeled UDP-Glc and unaffected by the inclusion of other phosphate-containing compounds, with the exception of UDP-galactose which can be converted to UDP-Glc by an endogenous epimerase.

• Research Organization: Duke Univ., Durham, NC (USA)
• OSTI ID: 5281594
• Resource Relation: Other Information: Thesis (Ph. D.)
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
PHOSPHOTRANSFERASES
ENZYME ACTIVITY
RECEPTORS
ATP
AUTORADIOGRAPHY
BIOCHEMICAL REACTION KINETICS
BRAIN
CHICKENS
ELECTROPHORESIS
EMBRYONIC CELLS
GLUCOPROTEINS
GLUCOSE
MOLECULAR WEIGHT
PHOSPHORUS 32
TRACER TECHNIQUES
ALDEHYDES
ANIMAL CELLS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIRDS
BODY
CARBOHYDRATES
CENTRAL NERVOUS SYSTEM
DAYS LIVING RADIOISOTOPES
ENZYMES
FOWL
HEXOSES
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LIGHT NUCLEI
MEMBRANE PROTEINS
MONOSACCHARIDES
NERVOUS SYSTEM
NUCLEI
NUCLEOTIDES
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANS
PHOSPHORUS ISOTOPES
PHOSPHORUS-GROUP TRANSFERASES
PROTEINS
RADIOISOTOPES
REACTION KINETICS
SACCHARIDES
TRANSFERASES
VERTEBRATES
550201* - Biochemistry- Tracer Techniques