Purification and partial characterization of a. beta. -glucan fragment that elicits phytoalexin accumulation in soybean
The synthesis of phytoalexins (antibiotics) in plant cells is induced by molecules called elicitors. Partial acid hydrolysis of mycelial walls of Phytophthora megasperma f. sp. glycinea solubilized a multicomponent mixture of elicitor-active and elicitor-inactive oligoglucosides. One elicitor-active and seven elicitor-inactive hexa(..beta..-D-glucopyranosyl)-D-glucitols were purified by gel-filtration, normal-phase partition, and reversed-phase liquid chromatography after reduction with NaBH/sub 4/. The elicitor-active and all but one of the elicitor-inactive hexa(..beta..-D-glucopyranosyl)-D-glucitols consisted of 3-, 6-, and 3,6-linked glucopyranosyl residues. The eighth hexa(..beta..-D-glucopyranosyl)-D-glucitol consisted of ..beta..-4-linked glucopyranosyl residues. The similarity of the structural characteristics of six of the elicitor-inactive hexa(..beta..-D-glucopyranosyl)-D-glucitols to the elicitor-active hexa(..beta..-D-glucopyranosyl)-D-glucitols suggested that a highly specific carbohydrate structure was required for elicitor activity. 47 references, 7 figures, 4 tables.
- Research Organization:
- Univ. of Colorado, Boulder
- DOE Contract Number:
- AC02-76ER01426
- OSTI ID:
- 5273064
- Journal Information:
- J. Biol. Chem.; (United States), Vol. 259:18
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
ANTIBIOTICS
BIOSYNTHESIS
GLYCINE HISPIDA
BIOLOGICAL PATHWAYS
ENZYME ACTIVITY
FUNGI
GENETICS
POLYSACCHARIDES
STRUCTURE-ACTIVITY RELATIONSHIPS
ANTI-INFECTIVE AGENTS
BACTERIA
BIOLOGY
CARBOHYDRATES
DRUGS
LEGUMINOSAE
MICROORGANISMS
ORGANIC COMPOUNDS
PLANTS
RHIZOBIUM
SACCHARIDES
SYNTHESIS
550200* - Biochemistry