Uptake of radiolabeled ions in normal and ischemia-damaged brain
The regional concentrations of nine radiochemicals were measured in rat brain after induction of cerebral ischemia to identify tracers concentrated by brain undergoing selective neuronal necrosis. Transient (30 minute) forebrain ischemia was produced in the rat; 24 hours after cerebral recirculation the radiochemicals were injected intravenously and allowed to circulate for 5 hours. The brain concentrations of the radiochemicals in dissected regions were determined by scintillation counting. Forebrain ischemia of this nature will produce extensive injury to striatal neurons but will spare the great majority of neocortical neurons at 24 hours. The regional concentrations of these radiochemicals varied considerably in both control and ischemic animals. In postischemic animals, 4 radionuclides (/sup 63/Ni, /sup 99/TcO/sub 4/, /sup 22/Na, and (/sup 3/H)tetracycline) were concentrated in the irreversibly damaged striatum in amounts ranging from 1.4 to 2.4 times greater than in normal tissue. The concentrations of /sup 65/Zn, /sup 59/Fe, /sup 32/PO/sub 4/, and /sup 147/Pm in postischemic brain were similar to or less than those in normal brain. The concentration of (14C)EDTA was increased in injured and uninjured brain of postischemic rats. Autoradiographic analysis of the distribution patterns of some of these ions in normal animals showed that /sup 99/TcO/sub 4/, /sup 22/Na, /sup 65/Zn, and /sup 59/Fe were distributed more uniformly throughout the brain than were /sup 32/PO/sub 4/, /sup 63/Ni, and /sup 147/Pm. At 24 or 48 hours after ischemia, /sup 63/Ni, /sup 99/TcO/sub 4/, and /sup 22/Na were preferentially concentrated in the damaged striatum and hippocampus, whereas /sup 65/Zn, /sup 59/Fe, /sup 32/PO/sub 4/, and /sup 147/Pm did not accumulate in irreversibly injured tissue. Of the radiochemicals tested to date, Ni, TcO/sub 4/, and tetracycline may be useful for diagnosing ischemic brain injury in humans, using positron emission tomography.
- Research Organization:
- Cornell Univ. Medical College, New York, NY
- OSTI ID:
- 5268672
- Journal Information:
- Ann. Neurol.; (United States), Vol. 5
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
62 RADIOLOGY AND NUCLEAR MEDICINE
BRAIN
RADIONUCLIDE KINETICS
TISSUE DISTRIBUTION
CARBON 14 COMPOUNDS
IRON 59
NICKEL 63
PHOSPHORUS 32
PROMETHIUM 147
SODIUM 22
TECHNETIUM 99
TRITIUM COMPOUNDS
ZINC 65
DIAGNOSIS
EDTA
ISCHEMIA
NECROSIS
RATS
SCINTILLATION COUNTING
ALKALI METAL ISOTOPES
AMINO ACIDS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BETA-PLUS DECAY RADIOISOTOPES
BODY
CARBOXYLIC ACIDS
CARDIOVASCULAR DISEASES
CENTRAL NERVOUS SYSTEM
CHELATING AGENTS
COUNTING TECHNIQUES
DAYS LIVING RADIOISOTOPES
DISEASES
DISTRIBUTION
ELECTRON CAPTURE RADIOISOTOPES
EVEN-ODD NUCLEI
HOURS LIVING RADIOISOTOPES
INTERMEDIATE MASS NUCLEI
IRON ISOTOPES
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
LABELLED COMPOUNDS
LIGHT NUCLEI
MAMMALS
NERVOUS SYSTEM
NICKEL ISOTOPES
NUCLEI
ODD-EVEN NUCLEI
ODD-ODD NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PATHOLOGICAL CHANGES
PHOSPHORUS ISOTOPES
PROMETHIUM ISOTOPES
RADIOISOTOPES
RARE EARTH ISOTOPES
RARE EARTH NUCLEI
RODENTS
SODIUM ISOTOPES
TECHNETIUM ISOTOPES
VASCULAR DISEASES
VERTEBRATES
YEARS LIVING RADIOISOTOPES
ZINC ISOTOPES
560172* - Radiation Effects- Nuclide Kinetics & Toxicology- Animals- (-1987)
550601 - Medicine- Unsealed Radionuclides in Diagnostics