Identification of a human transcription unit affected by the variant chromosomal translocations 2; 8 and 8; 22 of Burkitt lymphoma

Journal Article · Mon May 01 00:00:00 EDT 1989 · Proceedings of the National Academy of Sciences of the United States of America; (USA)

DOI:https://doi.org/10.1073/pnas.86.9.3257· OSTI ID:5263469

Shtivelman, E; Henglein, B; Groitl, P; Lipp, M; Bishop, J M ^[1]

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1. Univ. of California, San Francisco (USA)

Chromosomal translocations in Burkitt lymphoma and mouse plasmacytomas typically lie within or near the protooncogene MYC. In some instances, however, these tumors contain variant translocations with breakpoints located more distant from and downstream of MYC, in a domain commonly known as pvt-1. Until now, there has been no evidence that pvt-1 marks the location of a functional gene. Here the authors report the identification of a large transcriptional unit in human DNA that includes pvt-1. The authors have designated this unit as PVT. PVT begins 57 kilobase pairs downstream of MYC and occupies a minimum of 200 kilobase pairs of DNA. Some of the translocations that occur downstream of MYC in Burkitt lymphoma transect PVT; others lie between the two genes. None of the translocations they have studied appear to enhance transcription from an intact allele of PVT (indeed, they may inactivate that transcription), but some are associated with the production of abundant and anomalous 0.8- to 1.0-kilobase RNAs that contain the 5{prime} exon of PVT and sequences transcribed from the constant region of an immunoglobulin gene (the reciprocal participant in the translocation). Identification of PVT should facilitate the exploration of how translocations downstream of MYC and insertions of retroviral DNA in the vicinity of pvt-1 might contribute to tumorigenesis.

• OSTI ID: 5263469
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (USA), Vol. 86:9; ISSN 0027-8424
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
CHROMOSOMAL ABERRATIONS
TRANSCRIPTION
MESSENGER-RNA
AUTORADIOGRAPHY
ONCOGENES
DNA
DNA HYBRIDIZATION
DNA-CLONING
ELECTROPHORESIS
GENETIC MAPPING
HYBRIDIZATION
LYMPHOMAS
NEOPLASMS
PATIENTS
RECOMBINANT DNA
CLONING
DISEASES
GENES
IMMUNE SYSTEM DISEASES
MAPPING
MUTATIONS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
RNA
550400* - Genetics