Atrial natriuretic factor binding sites in experimental congestive heart failure

Journal Article · Sun Oct 01 00:00:00 EDT 1989 · American Journal of Physiology; (USA)

OSTI ID:5259840

Bianchi, C; Thibault, G; Wrobel-Konrad, E; De Lean, A; Genest, J; Cantin, M ^[1]

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1. Clinical Research Institute of Montreal, Quebec (Canada)

A quantitative in vitro autoradiographic study was performed on the aorta, renal glomeruli, and adrenal cortex of cardiomyopathic hamsters in various stages of heart failure and correlated, in some instances, with in vivo autoradiography. The results indicate virtually no correlation between the degree of congestive heart failure and the density of 125I-labeled atrial natriuretic factor ((Ser99, Tyr126)ANF) binding sites (Bmax) in the tissues examined. Whereas the Bmax was increased in the thoracic aorta in moderate and severe heart failure, there were no significant changes in the zona glomerulosa. The renal glomeruli Bmax was lower in mild and moderate heart failure compared with control and severe heart failure. The proportion of ANF B- and C-receptors was also evaluated in sections of the aorta, adrenal, and kidney of control and cardiomyopathic hamsters with severe heart failure. (Arg102, Cys121)ANF (des-(Gln113, Ser114, Gly115, Leu116, Gly117) NH2) (C-ANF) at 10(-6) M displaced approximately 505 of (Ser99, Tyr126)125I-ANF bound in the aorta and renal glomeruli and approximately 20% in the adrenal zona glomerulosa in both series of animals. These results suggest that ANF may exert a buffering effect on the vasoconstriction of heart failure and to a certain extent may inhibit aldosterone secretion. The impairment of renal sodium excretion does not appear to be related to glomerular ANF binding sites at any stage of the disease.

• OSTI ID: 5259840
• Journal Information: American Journal of Physiology; (USA), Vol. 257; ISSN 0002-9513
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
CARDIOVASCULAR DISEASES
PATHOGENESIS
PEPTIDES
RECEPTORS
ADRENAL GLANDS
AORTA
AUTORADIOGRAPHY
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL MODELS
COMPUTERIZED SIMULATION
HAMSTERS
IODINE 125
KIDNEYS
VASOCONSTRICTION
ANIMALS
ARTERIES
BETA DECAY RADIOISOTOPES
BLOOD VESSELS
BODY
CARDIOVASCULAR SYSTEM
DAYS LIVING RADIOISOTOPES
DISEASES
ELECTRON CAPTURE RADIOISOTOPES
ENDOCRINE GLANDS
GLANDS
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
ISOTOPES
KINETICS
MAMMALS
MEMBRANE PROTEINS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANS
PROTEINS
RADIOISOTOPES
REACTION KINETICS
RODENTS
SIMULATION
VERTEBRATES
550901* - Pathology- Tracer Techniques