Autoradiographic visualization of muscarinic receptors in human bronchi

Journal Article · Mon Feb 01 00:00:00 EST 1988 · J. Pharmacol. Exp. Ther.; (United States)

OSTI ID:5243651

van Koppen, C J; Blankesteijn, W M; Klaassen, A B; Rodrigues de Miranda, J F; Beld, A J; van Ginneken, C A

To visualize muscarinic receptors in human bronchi, the stripping film method was used which permits direct autoradiographic localization of tissue labeling. Cryostate sections of human bronchi were fixed in 0.5% glutaraldehyde in Krebs-Ringer buffer, pH 7.0 for 30 min at 0/sup 0/C, washed in Krebs-Ringer buffer for 20 min at 0/sup 0/C and incubated with (-)-(/sup 3/H)Quinuclidinyl benzilate ((-)-(/sup 3/H)QNB) for 90 min at 37/sup 0/C. Specific (-)-(/sup 3/H)QNB binding to tissue sections was saturable (receptor density of 0.14 +/- 0.03 fmol/tissue section) and of high affinity (Kd of 40 +/- 9 pM). For autoradiography, labeled tissue sections were covered with stripping film and exposed for 5 months. Muscarinic receptors in human bronchi were located predominantly in submucosal glands and parasympathetic ganglia. There was less labeling in smooth muscle cells and nerve bundles. Epithelium and blood vessels located within the bronchial wall were devoid of specific labeling.

• Research Organization: Univ. of Nijmegen (Netherlands)
• OSTI ID: 5243651
• Journal Information: J. Pharmacol. Exp. Ther.; (United States), Vol. 244:2
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
PARASYMPATHOMIMETICS
RECEPTORS
AUTORADIOGRAPHY
BIOLOGICAL LOCALIZATION
BIOCHEMICAL REACTION KINETICS
BLOOD VESSELS
BRONCHI
DOSE-RESPONSE RELATIONSHIPS
EPITHELIUM
MAN
TRITIUM COMPOUNDS
ANIMAL TISSUES
ANIMALS
AUTONOMIC NERVOUS SYSTEM AGENTS
BODY
CARDIOVASCULAR SYSTEM
DRUGS
KINETICS
LABELLED COMPOUNDS
MAMMALS
MEMBRANE PROTEINS
ORGANIC COMPOUNDS
ORGANS
PRIMATES
PROTEINS
REACTION KINETICS
RESPIRATORY SYSTEM
TISSUES
VERTEBRATES
550201* - Biochemistry- Tracer Techniques