Environmental effects on ethanol production by immobilized Zymomonas mobilis in a trickle-flow fermentor

Thesis/Dissertation · Fri Jan 01 00:00:00 EST 1988

OSTI ID:5233310

Lin, J J

A continuous trickle flow fermentor was successfully used to produce ethanol from glucose. Ethanol productivity of Z. mobilis ATCC 31821 was shown to produce ethanol about 2.5-fold faster and 38% higher than S. cerevisiae. A weighted equations was used to combine five parameters for the selection of a highly productive Z. mobilis NRRL 14022 which was used in the following experiment. The effect of environmental conditions (e.g., temperature, nutrient, initial pH, feed flow rate, ethanol and water activity) on fermentation and cell growth was investigated with the immobilized cell reactors. Based on reactor volume, ethanol productivity as high as 40.6 g/l/h with 90.7% conversion in an immobilized Z. mobilis reactor-separator (type I) has been obtained with 10% glucose and at 30{degree}C. Ethanol productivity of this system was found to be 71% higher than that of the immobilized cell reactor with no simultaneous ethanol separation. Cotton towel strip was used as a support to construct another immobilized cell rector-separator (type II). Reactor productivity was a linear function of inlet glucose concentration. This reactor was fed with 15% glucose and successfully operated for 41 days at 40{degree}C with no clogging problem as that occurred in the type I system.

• Research Organization: Purdue Univ., Lafayette, IN (USA)
• OSTI ID: 5233310
• Resource Relation: Other Information: Thesis (Ph. D.)
• Country of Publication: United States
• Language: English

Related Subjects

09 BIOMASS FUELS
ETHANOL
YIELDS
GLUCOSE
FERMENTATION
ZYMOMONAS MOBILIS
IMMOBILIZED CELLS
BIOREACTORS
GROWTH
NUTRIENTS
PH VALUE
TEMPERATURE DEPENDENCE
ALCOHOLS
ALDEHYDES
BACTERIA
BIOCONVERSION
CARBOHYDRATES
HEXOSES
HYDROXY COMPOUNDS
MICROORGANISMS
MONOSACCHARIDES
ORGANIC COMPOUNDS
SACCHARIDES
095000* - Biomass Fuels- Environmental Aspects- (1990-)
090900 - Biomass Fuels- Processing- (1990-)