Induction of highly immunogenic variants of Lewis lung carcinoma tumor by ultraviolet irradiation
This study was undertaken to determine whether in vitro treatment of Lewis lung carcinoma (3LL) cells with ultraviolet (UV) radiation could increase their immunogenicity. Tumor cells were irradiated with UV light from a germicidal lamp (254 nm; UV-C) at a dose of 720 J/sq m. After 2 weeks of culture, the surviving cell population was cloned by limiting dilution. Cell suspensions of each clone were injected intrafootpad in C57BL/6 mice at a dose of 2.5 X 10(5) cells per mouse. Eighty independent clones were tested. Fifty-one clones showed decreased tumorigenicity and failed to grow in 20 to 95% of immunocompetent mice, whereas they produced tumors in 100% of irradiated (550 R) and athymic nude mice. These clones were designated tum- (nontumorigenic) clones. In contrast, all 25 clones selected from the untreated parental 3LL induced progressively growing tumors in 100% of the mice. After two courses of UV treatment, the uncloned 3LL population was rejected in 45% of inoculated mice. Mice rejecting an inoculum of a tum- clone were completely resistant to subsequent challenge with higher doses of the same or unrelated tum- clones. This resistance was fully expressed even after irradiation of immune mice with 550 R. Mice immune to a tum- clone also were able to prevent the growth of various tum+ clones or untreated 3LL tumor cells. When tum- and tum+ clone cells were simultaneously inoculated intrafootpad in opposite legs, rejection of tum- clone resulted also in the prevention of the growth of tum+ clone. Spleen cells of immune mice caused rapid elimination of radiolabeled 3LL tumor cells from the place of their inoculation (intrafootpad) and prevented tumor growth.
- Research Organization:
- Frederick Cancer Research Facility, MD
- OSTI ID:
- 5228017
- Journal Information:
- Cancer Res.; (United States), Vol. 6
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
62 RADIOLOGY AND NUCLEAR MEDICINE
CARCINOMAS
RADIOTHERAPY
LUNGS
MICE
IMMUNE REACTIONS
TUMOR CELLS
BIOLOGICAL RADIATION EFFECTS
CELL CULTURES
CELL DIVISION
CLONE CELLS
CLONING
RADIOINDUCTION
SPLEEN
ULTRAVIOLET RADIATION
ANIMAL CELLS
ANIMALS
BIOLOGICAL EFFECTS
BODY
DISEASES
ELECTROMAGNETIC RADIATION
MAMMALS
MEDICINE
NEOPLASMS
NUCLEAR MEDICINE
ORGANS
RADIATION EFFECTS
RADIATIONS
RADIOLOGY
RESPIRATORY SYSTEM
RODENTS
THERAPY
VERTEBRATES
560121* - Radiation Effects on Cells- External Source- (-1987)
550603 - Medicine- External Radiation in Therapy- (1980-)