ELISA-Based Segregation of Adult Spring Chinook Salmon for Control of Bacterial Kidney Disease, Annual Report FY 1990.
Bacterial kidney disease (BKD), caused by Renibacterium salmoninarum (RS), is a serious disease of salmonid fish worldwide. The disease has a major impact on spring chinook salmon populations in the Columbia River system. There is strong evidence that RS can be transmitted from parent to progeny, and segregation of progeny based on levels of antigen detected in adult fish may obviate this mode of transmission. Results from the second year of a four year study to investigate segregation of broodstock as a tool for controlling BKD are presented. To segregate the progeny of adult fish infected with RS we have used enzyme-linked immunosorbent assays (ELISAs) optimized in the first year of this project. Gametes from fish either injected with erythromycin or receiving no antibiotic injection were successfully segregated into groups having either high or low levels of the RS soluble antigen. Screening of eggs from infected adults has not revealed any detectable antigen present in the egg tissue. Development of a rapid, field ELISA has been accomplished this year. The field ELISA utilizes monoclonal antibodies currently employed in the monoclonal antibody-based ELISA. The sensitivity of the field ELISA approaches that of the monoclonal ELISA, and has been tested on 150 adult chinook salmon. A high correlation exists between samples assayed by the monoclonal ELISA, field ELISA, and direct FAT. An alternative system for detecting RS soluble antigen, the Western blot, has also been improved. Using a chemiluminescent substrate, the sensitivity of detection has been increase 50--100 fold. 16 refs., 15 figs., 2 tabs.
- Research Organization:
- National Fisheries Research Center, Portland, OR (United States); Oregon State Univ., Corvallis, OR (United States)
- Sponsoring Organization:
- US Bonneville Power Administration
- DOE Contract Number:
- FG79-89BP95906
- OSTI ID:
- 5208467
- Report Number(s):
- DOE/BP-95906-2; ON: DE92000995
- Country of Publication:
- United States
- Language:
- English
Similar Records
ELISA-Based Segregation of Adult Spring Chinook Salmon for Control of Bacterial Kidney Disease, Annual Report FY 1989.
Umatilla Hatchery Satellite Facilities; Operations and Maintenance, Annual Report 2001.
Related Subjects
59 BASIC BIOLOGICAL SCIENCES
60 APPLIED LIFE SCIENCES
ANADROMOUS FISHES
UROGENITAL SYSTEM DISEASES
BACTERIA
INFECTIOUS DISEASES
ENVIRONMENTAL IMPACTS
MITIGATION
ENZYME IMMUNOASSAY
DIAGNOSTIC USES
OPTIMIZATION
PERFORMANCE TESTING
HYDROELECTRIC POWER
ANTIGENS
BACTERIAL DISEASES
ELECTROPHORESIS
ERYTHROMYCIN
GAMETES
MONOCLONAL ANTIBODIES
PROGRESS REPORT
SALMON
SCREENING
TROUT
ANIMALS
ANTI-INFECTIVE AGENTS
ANTIBIOTICS
ANTIBODIES
AQUATIC ORGANISMS
BIOASSAY
DISEASES
DOCUMENT TYPES
DRUGS
ELECTRIC POWER
ENERGY SOURCES
FISHES
GERM CELLS
IMMUNOASSAY
MICROORGANISMS
POWER
RENEWABLE ENERGY SOURCES
TESTING
USES
VERTEBRATES
Chinook salmon - Diseases - Columbia River Watershed
130600* - Hydro Energy- Environmental Aspects
550700 - Microbiology
553000 - Agriculture & Food Technology