Comparative mapping in the beige-satin region of mouse chromosome 13

Journal Article · Wed Jan 15 00:00:00 EST 1997 · Genomics

DOI:https://doi.org/10.1006/geno.1996.4478· OSTI ID:520408

Perou, C M; Pryor, R; Kaplan, J ^[1]

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1. Univ. of Utah School of Medicine, Salt Lake City, UT (United States); and others

The proximal end of mouse chromosome (Chr) 13 contains regions conserved on human chromosomes 1q42-q44, 6p23-p21, and 7p22-p13. This region also contains mutations that may be models for human disease, including beige (human Chediak-Higashi syndrome). An interspecific backcross of SB/Le and Mus spretus mice was used to generate a molecular genetic linkage map of mouse chromosome 13 with an emphasis on the proximal region including beige (bg) and satin (sa). This map provides the gene order of the two phenotypic markers bg and sa relative to restriction fragment length polymorphisms and simple sequence length polymorphisms in 131 backcross animals. In parallel, we have created a physical map of the region using Nidogen (Nid) as a molecular starting point for cloning a YAC contig that was used to identify the beige gene. The physical map provides the fine-structure order of genes and anonymous DNA fragments that was not resolved by the genetic linkage mapping. The results show that the bg region of mouse Chr 13 is highly conserved on human Chr 1q42-q44 and provide a starting point for a complete functional analysis of the entire bg-sa interval. 37 refs., 4 figs., 1 tab.

• OSTI ID: 520408
• Journal Information: Genomics, Vol. 39, Issue 2; Other Information: PBD: 15 Jan 1997
• Country of Publication: United States
• Language: English

Related Subjects

55 BIOLOGY AND MEDICINE
BASIC STUDIES
CHROMOSOMES
GENETIC MAPPING
COMPARATIVE EVALUATIONS
DNA SEQUENCING
DNA-CLONING
HUMAN CHROMOSOMES
GENES
GENE MUTATIONS
HEREDITARY DISEASES
ETIOLOGY
BIOLOGICAL MODELS
HUMAN POPULATIONS
PHENOTYPE
MICE
HUMAN CHROMOSOME 1
HUMAN CHROMOSOME 6
HUMAN CHROMOSOME 7
BIOLOGICAL MARKERS
RFLPS
CONTIGS
SOMATIC CELLS
HYBRIDIZATION