Changing the unstirred water layer in the intestine and its effect on absorption

Thesis/Dissertation · Fri Jan 01 00:00:00 EST 1988

OSTI ID:5186579

Lu, L

The purpose of this research was to examine possible methods for reducing the thickness of the unstirred water layer (UWL) in the canine intestinal lumen in vivo, and to determine the effects of any reduction obtained upon intestinal absorption. The experimental approaches employed in attempting to improve stirring of the luminal fluid include: (1) addition of oleic acid plus Na-taurocholate (OA + TC) or other bile salts to the fluid used to lavage the intestinal loops since the lavage with OA + TC has been found to increase the motility of the villi; (2) increasing the lavage flow rate to 100 ml/min; (3) introduction of air bubbles into the lavage fluid. The effect of these procedures on the UWL was determined by isotopic analysis of the tissue of the experimental intestinal segment for non-absorbable {sup 14}C-labeled inulin which was included in the lavage solutions. The effects of these procedures on intestinal absorption of water and glucose are examined by measuring the difference in the volumes and the concentrations of {sup 3}H-labeled glucose in the inflowing and outflowing fluids to the experimental segment.

• Research Organization: Minnesota Univ., Minneapolis, MN (USA)
• OSTI ID: 5186579
• Resource Relation: Other Information: Thesis (Ph. D.)
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
GLUCOSE
INTESTINAL ABSORPTION
WATER
CARBON 14 COMPOUNDS
INULIN
LAVAGE
OLEIC ACID
TRACER TECHNIQUES
TRITIUM COMPOUNDS
ABSORPTION
ALDEHYDES
CARBOHYDRATES
CARBOXYLIC ACIDS
HEXOSES
HYDROGEN COMPOUNDS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MONOCARBOXYLIC ACIDS
MONOSACCHARIDES
ORGANIC ACIDS
ORGANIC COMPOUNDS
OXYGEN COMPOUNDS
POLYSACCHARIDES
SACCHARIDES
UPTAKE
550501* - Metabolism- Tracer Techniques