Characterization of the phosphatidylinositol-glycan membrane anchor of human placental alkaline phosphatase
Placental alkaline phosphatase (orthophosphoric-monoester phosphohydrolase (alkaline optimum), EC 3.1.3.1) is a member of a diverse group of membrane proteins whose attachment to the lipid bilayer is mediated by a phosphatidylinositol-glycan. To investigate structural aspects of the glycolipid anchor, cultured WISH cells were used because, they produce the enzyme in abundant quantities. When cell suspensions were incubated with purified phosphatidylinositol-specific phospholipase C, most of the placental alkaline phosphatase was released from membranes in a hydrophilic form. On incubation of the cells with (/sup 14/C)ethanolamine, (/sup 14/C)myristic acid, or myo(/sup 3/H)inositol, each was incorporated into the phosphatase near the carboxyl terminus, showing that these components, which are found in other phosphatidylinositol membrane-linked proteins, are also present in placental alkaline phosphatase.
- Research Organization:
- Roche Research Center, Nutley, NJ (USA)
- OSTI ID:
- 5175508
- Journal Information:
- Proc. Natl. Acad. Sci. U.S.A.; (United States), Vol. 84:17
- Country of Publication:
- United States
- Language:
- English
Similar Records
Phosphatidylinositol anchor of HeLa cell alkaline phosphatase
Phospholipase D specific for the phosphatidylinositol anchor of cell-surface proteins is abundant in plasma
Related Subjects
ALKALINE PHOSPHATASE
BIOCHEMISTRY
GLYCOLIPIDS
BIOSYNTHESIS
LABELLED COMPOUNDS
AMINES
CARBON 14 COMPOUNDS
CELL MEMBRANES
ELECTROPHORESIS
HYBRIDIZATION
INOSITOLS
LIQUID COLUMN CHROMATOGRAPHY
MESSENGER-RNA
METHIONINE
PLACENTA
SOMATIC CELLS
SULFUR 35
TETRADECANOIC ACID
TRITIUM COMPOUNDS
AMINO ACIDS
ANIMAL CELLS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CARBOHYDRATES
CARBOXYLIC ACIDS
CELL CONSTITUENTS
CHEMISTRY
CHROMATOGRAPHY
DAYS LIVING RADIOISOTOPES
DRUGS
ENZYMES
ESTERASES
EVEN-ODD NUCLEI
FETAL MEMBRANES
HYDROLASES
ISOTOPES
LIGHT NUCLEI
LIPIDS
LIPOTROPIC FACTORS
MEMBRANES
MONOCARBOXYLIC ACIDS
MONOSACCHARIDES
NUCLEI
NUCLEIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PHOSPHATASES
RADIOISOTOPES
RNA
SACCHARIDES
SEPARATION PROCESSES
SULFUR ISOTOPES
SYNTHESIS
550201* - Biochemistry- Tracer Techniques