Chromosome region-specific libraries for human genome analysis

Technical Report · Tue Jan 01 00:00:00 EST 1991

DOI:https://doi.org/10.2172/5163343· OSTI ID:5163343

Kao, Fa-Ten

We have made important progress since the beginning of the current grant year. We have further developed the microdissection and PCR- assisted microcloning techniques using the linker-adaptor method. We have critically evaluated the microdissection libraries constructed by this microtechnology and proved that they are of high quality. We further demonstrated that these microdissection clones are useful in identifying corresponding YAC clones for a thousand-fold expansion of the genomic coverage and for contig construction. We are also improving the technique of cloning the dissected fragments in test tube by the TDT method. We are applying both of these PCR cloning technique to human chromosomes 2 and 5 to construct region-specific libraries for physical mapping purposes of LLNL and LANL. Finally, we are exploring efficient procedures to use unique sequence microclones to isolate cDNA clones from defined chromosomal regions as valuable resources for identifying expressed gene sequences in the human genome. We believe that we are making important progress under the auspices of this DOE human genome program grant and we will continue to make significant contributions in the coming year. 4 refs., 4 figs.

• Research Organization: Eleanor Roosevelt Inst. for Cancer Research, Denver, CO (United States)
• Sponsoring Organization: USDOE; USDOE, Washington, DC (United States)
• DOE Contract Number: FG02-91ER61139
• OSTI ID: 5163343
• Report Number(s): DOE/ER/61139-1; ON: DE92001741
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
CHROMOSOMES
DNA SEQUENCING
DNA-CLONING
MAN
GENETIC MAPPING
BANDING TECHNIQUES
BIOCHEMISTRY
DNA
DNA HYBRIDIZATION
DNA POLYMERASES
ELECTROPHORESIS
ENDONUCLEASES
HUMAN CHROMOSOME 21
MICROSCOPY
MOLECULAR BIOLOGY
OLIGONUCLEOTIDES
PLASMIDS
PROBES
PROGRESS REPORT
RNA
ANIMALS
CELL CONSTITUENTS
CHEMISTRY
CLONING
CYTOLOGICAL TECHNIQUES
DNA-ASE
DOCUMENT TYPES
ENZYMES
ESTERASES
HYBRIDIZATION
HYDROLASES
MAMMALS
MAPPING
NUCLEIC ACIDS
NUCLEOTIDYLTRANSFERASES
ORGANIC COMPOUNDS
PHOSPHODIESTERASES
PHOSPHORUS-GROUP TRANSFERASES
POLYMERASES
PRIMATES
STRUCTURAL CHEMICAL ANALYSIS
TRANSFERASES
VERTEBRATES
550200* - Biochemistry
550400 - Genetics