Polarized neutron scattering from dynamically polarized targets in biology

Journal Article · Wed Apr 01 00:00:00 EST 1992 · Nuclear Science and Engineering; (United States)

OSTI ID:5141427

Knop, W; Stuhrmann, H B; Wagner, R; Wenkow-EsSouni, M; Zhao, J ^[1]; Schaerpf, O ^[2]; Krumpolc, M ^[3]; Nierhaus, K H ^[4]; Niinikoski, T O; Rijllart, A ^[5]

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1. GKSS Forschungszentrum, W-2054 Geesthacht (DE)
2. Inst. Laue-Langevin, F-38042 Grenoble (FR)
3. Univ. of Illinois at Chicago, Dept. of Chemistry, Chicago, IL (US)
4. Max-Planck-Inst. fuer Molekular Genetik, Berlin (DE)
5. European Organization for Nuclear Research, PPE, CH-1211 Geneva (CH)

This paper discusses polarized neutron scattering from clusters of polarized proton spins in solid material which provides a new contrast variation method. Frozen solutions of apoferritin and of the large subunit of Escherichia coli ribosomes in a mixture of heavy water and deuterrated glycerol hae been studied at the conditions of dynamic nuclear spin polarization (H = 2.5 T, T {lt} 1 K, 4-mm microwave irradiation). The three basic scattering functions of contrast variation were derived by varying polarized neutron scattering with the polarization of target nuclei. They agree with results obtained form neutron scattering in H{sub 2}O/D{sub 2}O mixtures at room temperature. Furthermore, the proton spins appear to be polarized uniformly, at least to a structural resolution of 40 {Angstrom}. This is an important prerequisite for the in situ structure determination of macromolecular labels in larger host particles.

• OSTI ID: 5141427
• Journal Information: Nuclear Science and Engineering; (United States), Vol. 110:4; ISSN 0029-5639
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
75 CONDENSED MATTER PHYSICS
SUPERCONDUCTIVITY AND SUPERFLUIDITY
POLARIZED TARGETS
NEUTRON DIFFRACTION
BINARY MIXTURES
BIOLOGY
ESCHERICHIA COLI
GLYCEROL
HEAVY WATER
POLARIZED BEAMS
PROTONS
RESOLUTION
SOLIDS
SPIN
ALCOHOLS
ANGULAR MOMENTUM
BACTERIA
BARYONS
BEAMS
COHERENT SCATTERING
DIFFRACTION
DISPERSIONS
ELEMENTARY PARTICLES
FERMIONS
HADRONS
HYDROGEN COMPOUNDS
HYDROXY COMPOUNDS
MICROORGANISMS
MIXTURES
NUCLEONS
ORGANIC COMPOUNDS
OXYGEN COMPOUNDS
PARTICLE PROPERTIES
SCATTERING
TARGETS
WATER
550700* - Microbiology
665100 - Nuclear Techniques in Condensed Matter Physics - (1992-)