Nonphotochemical hole burning of the reaction center of Rhodopseudomonas viridis
- Iowa State Univ., Ames, IA (United States)
Reddy et al. (Science, accepted) have reported persistent, nonphotochemical hole-burned (NPHB) spectra for the Q[sub y] states of the reaction center of Rhodopseudomonas viridis. The photoinduced structural transformation was shown to be highly localized on the special pair. This transformation leads to a red shift of the special pair's lowest-energy absorption band, P960, of 150 cm[sup [minus]1] and a comparable blue shift for a state at 850 nm, which, as a consequence, could be assigned as being most closely associated with the upper dimer component. Additional experimental results are presented here together with a theoretical analysis of the extent to which the NPHB spectra provide information on the contribution from the bacteriochlorophyll monomers of the special pair to the Q[sub y] states that absorb higher in energy than P960. Structured photochemical hole-burned (PHB) spectra of P960 are also presented that underscore the importance of strong electron-phonon coupling from a broad distribution of modes with a mean frequency of 30 cm[sup [minus]1] for an understanding of the P960 absorption profile. These spectra also identify the zero-phonon hole of the strongly damped special pair marker mode (145 cm[sup [minus]1]) and its associated phonon sideband structure. Calculated spectra are presented which are in good agreement with the experimental PHB spectra. 30 refs., 6 figs., 4 tabs.
- DOE Contract Number:
- W-7405-ENG-82
- OSTI ID:
- 5136106
- Journal Information:
- Journal of Physical Chemistry; (United States), Vol. 97:26; ISSN 0022-3654
- Country of Publication:
- United States
- Language:
- English
Similar Records
Spectroscopic properties of photosynthetic reaction centers. 2. Application of the theory to Rhodopseudomonas viridis
Strong acceleration of primary photosynthetic electron transfer in a mutated reaction center of Rhodopseudomonas viridis
Related Subjects
37 INORGANIC
ORGANIC
PHYSICAL AND ANALYTICAL CHEMISTRY
PHOTOSYNTHETIC BACTERIA
CHEMICAL REACTIONS
PROTEINS
ABSORPTION SPECTROSCOPY
COMPILED DATA
ELECTRON TRANSFER
ELECTRON-PHONON COUPLING
HOLES
MOLECULAR STRUCTURE
BACTERIA
COUPLING
DATA
INFORMATION
MICROORGANISMS
NUMERICAL DATA
ORGANIC COMPOUNDS
SPECTROSCOPY
550200* - Biochemistry
400500 - Photochemistry