Transport of macromolecules and particles at target sites for deposition of air pollutants
- Univ. of California, Irvine (USA)
This study analyzed rats' nasal, tracheal and bronchoalveolar epithelial permeability to macromolecules after they were exposed, in 2- or 4-hour periods of rest or exercise, to ozone (O3) (0.6, 0.8 or 2 ppm), nitrogen dioxide (NO2) (2.5, 6 or 12 ppm) or formaldehyde (10 ppm). Exercise was performed on a treadmill operated at a speed that led to a 2-fold increase in oxygen consumption. Histopathologic and electron microscopic cytochemical and autoradiographic studies were performed to identify the structural aspects of mucosal response. In rats not exposed to pollutants, the quantity of macromolecular tracers (99mTc-DTPA, 125I-BSA) in blood sampled 6, 7, 8, 9 and 10 minutes after a slow 5-minute instillation of comparable quantities of tracer molecules in the lumen of each zone, was lowest in nasal, highest in tracheal, and intermediate in the bronchoalveolar region. Exposure of resting rats to O3 did not affect nasal permeability, but tracheal and bronchoalveolar permeabilities increased by 2-fold 1 hour after the exposure. In rats exposed at rest to O3, tracheal permeability was no longer elevated 24 hours after exposure, but bronchoalveolar permeability remained elevated at 24 hours after exposure and was normal at 48 hours. Exposure during exercise increased the effect of O3 in the trachea and in the bronchoalveolar zone. However, exercise also prolonged the duration of the O3 effect on the tracheal zone from 1 hour to 24 hours and, in the bronchoalveolar zone, from 24 hours to 48 hours. Histologically, focal inflammatory lesions in the alveolar zone were maximal at 48 hours after a 4-hour resting exposure to O3. After exposure during exercise, the area of lung involved by lesions increased 4- to 7-fold above the lesion-bearing area in rats exposed while resting.
- OSTI ID:
- 5127989
- Journal Information:
- Research Report Health Effects Institue; (USA), Vol. 3
- Country of Publication:
- United States
- Language:
- English
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59 BASIC BIOLOGICAL SCIENCES
CELL MEMBRANES
PERMEABILITY
FORMALDEHYDE
TOXICITY
NITROGEN DIOXIDE
OZONE
AIR POLLUTION
AUTORADIOGRAPHY
DTPA
ELECTRON MICROSCOPY
EPITHELIUM
EXERCISE
IODINE 125
ISOMERIC NUCLEI
MEMBRANE TRANSPORT
PATHOLOGICAL CHANGES
RATS
RESPIRATORY SYSTEM
TECHNETIUM 99
ALDEHYDES
AMINO ACIDS
ANIMAL TISSUES
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BODY
CARBOXYLIC ACIDS
CELL CONSTITUENTS
CHALCOGENIDES
CHELATING AGENTS
DAYS LIVING RADIOISOTOPES
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
HOURS LIVING RADIOISOTOPES
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
MAMMALS
MEMBRANES
MICROSCOPY
NITROGEN COMPOUNDS
NITROGEN OXIDES
NUCLEI
ODD-EVEN NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
OXIDES
OXYGEN COMPOUNDS
POLLUTION
RADIOISOTOPES
RADIOPROTECTIVE SUBSTANCES
RODENTS
TECHNETIUM ISOTOPES
TISSUES
VERTEBRATES
YEARS LIVING RADIOISOTOPES
560300* - Chemicals Metabolism & Toxicology
550501 - Metabolism- Tracer Techniques