Induction of sister chromatid exchange in preimplantation mouse embryos in vitro by /sup 3/H-thymidine or ultraviolet light in combination with caffeine
Preimplantation mouse embryos were exposed in vitro to /sup 3/H-thymidine (25, 100, or 250 Bq/ml) or ultraviolet (UV) light (1.35 or 4.05 J/m2), either alone or in combination with caffeine (1 mM with /sup 3/H-thymidine and 0.5 mM with UV light). Exposure to /sup 3/H-thymidine lasted for 2 days, from the two-cell stage to the late morula/early blastocyst stage, and UV radiation was applied acutely at the late morula/early blastocyst stage. The effects were quantified by the sister chromatid exchange (SCE) assay. All three agents induced SCEs when used singly. /sup 3/H-thymidine was effective in inducing SCEs only at 250 Bq/ml, whereas UV light was effective at both fluences. Although caffeine did not induce SCEs when it was added before exposure to bromodeoxyuridine (BrdUrd), which is used to visualize SCEs, it did induce SCEs when present during the entire culture period (/sup 3/H-thymidine experiments) or during incubation in BrdUrd (UV experiments). Caffeine markedly enhanced the SCE-inducing effect of UV light but did not influence the effect of /sup 3/H-thymidine.
- Research Organization:
- Univ. of California, San Francisco
- OSTI ID:
- 5120376
- Journal Information:
- Teratog., Carcinog., Mutagen.; (United States), Vol. 2
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
CAFFEINE
GENETIC EFFECTS
TRITIUM COMPOUNDS
ULTRAVIOLET RADIATION
EMBRYOS
IN VITRO
MICE
SISTER CHROMATID EXCHANGES
SYNERGISM
THYMIDINE
TOXICITY
ANALEPTICS
ANIMALS
AROMATICS
AZAARENES
AZINES
BIOLOGICAL EFFECTS
CENTRAL NERVOUS SYSTEM AGENTS
CHROMOSOMAL ABERRATIONS
DRUGS
ELECTROMAGNETIC RADIATION
HETEROCYCLIC COMPOUNDS
LABELLED COMPOUNDS
MAMMALS
MUTATIONS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
PURINES
PYRIMIDINES
RADIATIONS
RIBOSIDES
RODENTS
VERTEBRATES
XANTHINES
560122* - Radiation Effects on Cells- Internal Source- (-1987)
560121 - Radiation Effects on Cells- External Source- (-1987)